2种色型黄粉虫酚氧化酶同工酶的比较  

Comparison of phenoloxidase isozymes from two color varieties of Tenebrio molitor L.

作　　者：黄琼[1,2] 胡杰[3] 周定刚[4] 苟琳[5] 

机构地区：[1]四川农业大学森林保护学省级重点实验室,四川雅安625014 [2]四川农业大学长江上游林业生态工程四川省重点实验室,四川雅安625014 [3]四川农业大学经济管理学院,四川成都611130 [4]四川农业大学动物科技学院,四川雅安625014 [5]四川农业大学生命科学与理学院生物系,四川雅安625014

出　　处：《浙江大学学报（农业与生命科学版）》2012年第4期377-386,共10页Journal of Zhejiang University:Agriculture and Life Sciences

基　　金：四川省教育厅资助项目(10ZA056;07ZZ022);国家级大学生创新性实验计划资助项目(101062624)

摘　　要：为黄粉虫(Tenebrio molitor L.)品种选育、体色分化及免疫防御研究提供参考,采用不连续聚丙烯酰胺凝胶垂直板电泳并结合分光光度法,对黄、黑2种色型黄粉虫各发育期的酚氧化酶(包括单酚氧化酶和二酚氧化酶)同工酶酶谱及酶活性进行比较研究。结果表明:在2种色型黄粉虫的各发育期,共发现迁移率不同的单酚氧化酶(MPO)同工酶酶带6条、二酚氧化酶(DPO)同工酶酶带5条,其中MPO酶带分布在3个泳动区,DPO酶带分布在2个泳动区,黑色型卵比黄色型卵多1条MPO特征性酶带;并且黑色型卵、幼虫和成虫的MPO和DPO活性显著高于黄色型卵、幼虫和成虫;但在蛹期,MPO活性黄色型比黑色型高,而DPO活性则是黑色型比黄色型高;此外,同一色型黄粉虫在不同发育阶段的MPO和DPO同工酶酶带数、酶活性强弱及迁移率变化明显,其中,卵期有MPO和DPO特有酶带各1条,幼虫期只有1条DPO特有酶带;同时,各发育阶段的MPO和DPO同工酶酶谱及酶活性变化规律不同,由卵至成虫期,MPO活性变化趋势为:卵期>蛹期>成虫期>幼虫期,DPO活性变化趋势为:幼虫期>成虫期>蛹期>卵期。上述结果为进一步研究黄粉虫的体色分化、免疫防御和品种选育奠定了理论基础。In order to provide some references for studying the body-color diversity, immune defence and variety selection of Tenebrio rnolitor, the phenoloxidase （including monophenol oxidase and diphenol oxidase） isozyme zymograms and activity at different developmental stages of the yellow- and black-color varieties of T. molitor were compared using vertical polyacrylamide gel electrophoresis and spectrophotometry. The results showed that, during their developmental stages, six monophenol oxidase （MPO） bands and five diphenol oxidase （DPO） bands with different mobility were found, in which the MPO bands were distributed in three zones, while the DPO bands were in two zones. Meanwhile, the eggs of the black-color variety showed one more distinctive MPO bands compared with those of the yellow-color variety. Moreover, the MPO and DPO activities of black-color variety eggs, larvae and adults were significantly higher than those of the yellow-color ones. But at the pupal stage, the MPO activity of the yellow-color variety was higher than that of the black-color one, while the DPO activity was opposite. Distinctive variations were shown in the band numbers, migration rate of enzyme bands and strength of enzymatic activity of MPO and DPO at different developmental stages of both color varieties. Compared with other developmental stages, there was one MPO and one DPO characteristic enzyme band at egg stage of both color varieties, but only one typical DPO band at larval stage. Meanwhile, there was different change on the zymograms and the activities of MPO and DPO of both color varieties at different developmental stages. From the egg to adult stage of both color varieties, the MPO activity changed in the descending order of egg stage 〉 pupal stage 〉 adult stage 〉 larval stage, while the DPO activity varied in the descending order of larval stage 〉 adult stage 〉 pupal stage 〉 egg stage. The results above lay a theoretical foundation for further research on the body-color diversity, immune defence and var

关 键 词：黄粉虫 黄色型 黑色型 单酚氧化酶 二酚氧化酶 酶谱 酶活性 

分 类 号：Q969.97[生物学—昆虫学] Q965