古尼拟青霉最佳镇痛活性培养基的筛选  

Medium Screening for the Best Analgesic Activity of Paecilomyces gunnii

作　　者：胡拓[1,2] 朱国胜[1,2] 刘永翔[1,2] 刘作易[1,2] 

机构地区：[1]贵州省农业生物技术重点实验室,贵阳550006 [2]贵州省农业科学院,贵阳550006

出　　处：《菌物研究》2012年第3期201-204,共4页Journal of Fungal Research

基　　金：贵州省农业科学院课题"古尼虫草活性成分研究"[院ZX(2007)035号]

摘　　要：以1株有镇痛活性的古尼拟青霉为试验材料,从5种培养基中筛选出使该株产生最佳镇痛活性成分的培养基。结果表明:产生镇痛活性成分的最佳发酵时间为6 d,最佳培养基为改良沙氏培养基Ⅰ,其成分为葡萄糖20 g,甘油5 g,可溶性淀粉5 g,KCl 0.5 g,MgSO4.7H2O 0.5 g,KH2PO4.3H2O 1 g,FeSO4.7H2O 0.5 g,pH 7～8。A strain of Paecilomyces gunnii was used as test material to select the optimum medium among 5 media in which the best analgesic activity composition could be produced. The results show that the op- timal medium is the Sabouraud improving medium Ⅰ with pH 7--8, which contains dextrose 20 g, glyc- erol 5 g, soluble starch 5 g, KCl 0.5 g, MgSO4·7H2O 0.5 g, KH2PO4·3H2O 1 g, and FeSO4·7H2O 0.5 g. The optimum fermentation time is 6 days.

关 键 词：古尼拟青霉 镇痛活性 最佳培养基 

分 类 号：S567.3[农业科学—中草药栽培]