不同发育特性小麦品种春化基因VRN2的序列分析  

Sequences Analysis of the Vernalization Gene VRN2 in Different Development Characteristic Common Wheat(Triticum aestivum L.)

作　　者：马丽娟[1] 王翔[1] 卫丽[1] 冯雅岚[1] 任江萍[1] 尹钧[1] 

机构地区：[1]河南农业大学,国家小麦工程技术研究中心,河南郑州450002

出　　处：《麦类作物学报》2012年第4期603-609,共7页Journal of Triticeae Crops

基　　金：国家支撑计划重大项目(2011BAD16B07);河南省教育厅自然基金项目(2010B210015)

摘　　要：为了明确小麦春化基因VRN2与春化发育表现型的关系,以6个不同春化发育特性的普通小麦品种为试验材料,采用PCR技术对春化基因VRN2的CCT保守区中43个氨基酸序列进行了分析。结果表明,不同品种间ZCCT-A1的CCT功能域的序列存在差异,肥麦有R35W突变,另外5个品种均有R39C突变;ZCCT-A2均存在R16C突变;B和D基因组中均未发现突变。这表明VRN2基因编码区的等位变异主要出现在A基因组上,而B和D基因组中的VRN2基因在目前大面积主栽品种中均为显性。To identify the relationship of vernalization gene VRN2 and growth habit in common wheat, The 43 amino acid sequences of the conserved CCT domain in the ZCCT1 and ZCCT2 genes of 6 wheat （Triticum aestivum） cultivars with different vernalization traits were analyzed using the sequence-spe- cific PCR amplification. The results showed that 6 cultivars had different mutation in CCT domain of ZCCT-AJ gene, Feimai had R35W mutation, the other five cultivars had R39C mutation inZCCT-A1 ~ while all of the 6 cultivars had R16C mutation inZCCT-A2 gene. B and D genomes of the 6 cultivars had no mutations Allelic variation of VRN2 coding region is mainly reflected in the A genome, while VRN2 in B,D genome is dominant in main cultivars.

关 键 词：普通小麦 春化基因VRN2 突变 

分 类 号：S512.1[农业科学—作物学] S330