Prediction on Antigenic Epitope Characteristics of Bt Cry2Ab Protein in Transgenic Crops  

转基因植物中Bt Cry2Ab蛋白的抗原表位特征预测(英文)

作　　者：高洁荣[1,2] 何颖[2] 邹泽红[2] 陶爱林[2] 艾云灿[1] 

机构地区：[1]中山大学生命科学院/有害生物控制与资源利用国家重点实验室,广东广州510275 [2]广州医学院第二附属医院/呼吸疾病国家重点实验室变态反应研究室/过敏反应与临床免疫重点实验室,广东广州510260

出　　处：《Agricultural Science & Technology》2012年第7期1493-1496,1582,共5页农业科学与技术（英文版）

基　　金：国家科技重大专项重大课题(2008ZX08011-005)及重点课题(2009ZX08011-004B);国家自然基金(30771240);呼吸疾病国家重点实验室(广州医学院)课题研究基金共同资助~~

摘　　要：[Objective] This study aimed to predict the structural characteristics of Bt Cry2Ab protein in transgenic crops with bioinformatic analysis to provide the theoretical clues for design of antibody Cry2Ab. [Method] The amino acid sequence of Cry2Ab protein was searched from NCBI database. The B cell epitopes were predicted with DNAStar. The binding affinity between Cry2Ab protein and MHC-II molecules was analyzed with NetMHCII 2.2 Server to predict the T cell epitopes. [Result] Prediction result suggested the potential B cell epitope of Cry2Ab locating in the region of 208-215. Analysis of the binding affinity between Cry2Ab and MHC-II molecules suggested the regions of 177-185, 299-307 and 255-263 were the potential T cell epitopes. Human with HLA-DRB10101 alleles and HLA-DRB10701 alleles were more sensitive to Cry2Ab protein. [Conclusion] This study facilitates to understand the structural characteristics of Cry2Ab protein and provides a new clue to improve the assessment method for potential allergenicity of genetically modified food.[目的]通过生物信息学方法了解转基因作物中BtCry2Ab蛋白的二级结构、三级结构、B细胞抗原表位及T细胞抗原表位等结构特征,为今后的抗体设计奠定基础。[方法]以在NCBI数据库中获得Cry2Ab蛋白序列为材料,应用DNAStar中多种方法预测其B细胞表位,并通过在线预测软件NetMHCII2.2分析Cry2Ab蛋白与MHC-II类分子的结合能力从而预测其T细胞抗原表位。[结果]对Cry2Ab蛋白的B细胞抗原表位的预测表明,Cry2Ab蛋白的第208～215区域是潜在的B细胞抗原表位。对Cry2Ab蛋白与MHC-II类分子结合能力的分析表明,Cry2Ab蛋白的第177～185区域、299～307区域和255～263区域是潜在的T细胞抗原表位,暗示含有HLA-DRB10101和HLA-DRB10701等位基因的人群对Cry2Ab蛋白更敏感。[结论]该研究有助于深入了解Cry2Ab蛋白的生物学特征,为完善转基因食品过敏原性的评估方法提供新线索。

关 键 词：Transgenic crops ALLERGENICITY CRY2AB B cell epitopes T cell epitopes 

分 类 号：Q943.2[生物学—植物学]