Forsythiaside A Induced IFN-α and Up-regulated the Correlated Factors in JAK-STAT Signaling Pathway in vitro  

连翘酯苷在体外对鸡IFN-α和JAK-STAT信号通路相关因子的影响(英文)

作　　者：李华伟[1] 张中文[1] 陆彦[1] 马元元[2] 张禹 吴国娟[1] 

机构地区：[1]北京农学院动物科技学院,北京102206 [2]北京大学第一医院动物室,北京100034 [3]北京勤邦生物技术有限公司,北京102206

出　　处：《Agricultural Science & Technology》2012年第7期1566-1570,共5页农业科学与技术（英文版）

基　　金：国家自然科学基金(31172362,30972215);北京市自然科学基金(5102014,6092004);北京市属高校人才强教计划(PHR)~~

摘　　要：[Objective] This study was to investigate the effect of forsythiaside A added in chick embryo kidney cells (CEKs), on the changes in expression of interferon α(IFN-α) and mRNA expression level of correlated factors in JAK-STAT pathway. [Methods] Three levels of forsythiaside (100, 200, 400 μg/ml) were adopted to treat the experimental materials, then the expression levels of IFN-α and correlated factors in JAK-STAT pathway were detected by real-time RT-PCR at the transcriptional level (mRNA) and by Western blot at the translation level(IFN-α protein). [Result] Compared with the control group (untreated group), Application of forsythiaside A not only significantly increased the expression of IFN-α in treated CEKs (P<0.05) , but also up-regulated the expression of STAT1, JAK1, IFNAR1, IFNAR2, IRF1 and IRF7, the correlated factors in JAK-STAT pathway. [Conclusion] Forsythiaside A induced the expression of IFN-α in CEKs, and can positively regulate the JAK-STAT signaling pathway significantly.[目的]探讨连翘酯苷作用于鸡胚肾(CEK)细胞后,IFN-α的表达变化及JAK-STAT通路相关因子mRNA的表达变化,及连翘酯苷对IFN-α和JAK-STAT信号通路的影响。[方法]将连翘酯苷设为3个浓度(100、200和400μg/ml),采用荧光定量RT-PCR,检测IFN-α和JAK-STAT通路相关因子的mRNA表达变化;用western blotting检测IFN-α的蛋白表达情况。[结果]与正常组相比,连翘酯苷不仅显著提高了IFN-α的表达量(P<0.05);而且明显上调了STAT1、JAK1、IFNAR1、IFNAR2、IRF1、IRF7的表达。[结论]连翘酯苷能够在鸡胚肾细胞(chicken embryo kidney cells,CEKs)上诱导IFN-α的表达,且能够正向调节JAK-STAT信号通路传导。

关 键 词：Forsythoside A Chick embryo kidney cells IFN-Α JAK-STAT Real-time PCR Western blot 

分 类 号：S831[农业科学—畜牧学]