酪蛋白磷酸肽-非结晶型磷酸钙与含氟制剂抑制牙釉质脱矿的比较研究  

Comparative studies of casein phosphopeptide-amorphous calcium phosphate and fluoride products in inhibiting enamel demineralization

作　　者：张春波[1] 孙新华[1] 侯旭[1] 闫阔[1] 

机构地区：[1]吉林大学口腔医学院正畸科,长春130021

出　　处：《中华口腔医学杂志》2012年第8期490-494,共5页Chinese Journal of Stomatology

摘　　要：目的评价酪蛋白磷酸肽-非结晶型磷酸钙（casein phosphopeptide—amorphous calcium phosphate，CPP-ACP）与几种临床常用的含氟制剂抑制牙釉质脱矿的作用，为正畸临床预防牙釉质脱矿提供依据。方法将70颗离体牙分为7组，每组10颗，即A组：CPP—ACP组；B组：CPP—ACP＋含氟漱口水组；C组：含氟漱口水组；D组：含氟玻璃离子牙体保护剂组；E组：含氟树脂粘接剂组；F组：氟保护漆组；G组：空白对照组。将7组样本按三餐进食时间放入人工致龋液中，每次10min，前6组分别于样本颊面釉质表面涂布CPP-ACP、CPP-ACP和含氟漱口水、含氟漱口水、含氟玻璃离子牙体保护剂、含氟树脂粘接剂、氟保护漆，空白对照组不处理，7组样本其余时间均浸泡于人工唾液中。于实验1、2、3个月用原子力显微镜检测。结果实验1个月D组釉质表面粗糙度[（114±1）nm]显著低于G组[（172±9）nm]（P〈0．05）；实验2和3个月各组釉质表面粗糙度与G组差异均有统计学意义（P〈0．05）；B组粗糙度2和3个月为（87±9）和（27±6）nm，显著低于其他各组（P〈0．05）；C组2和3个月粗糙度分别为（145±8）和（126±7）nm，显著高于D、E、F组（P〈0．05）。结论CPP—ACP与含氟制剂在人工致龋环境中均有抑制牙釉质脱矿的作用，CPP-ACP与含氟制剂联合应用效果更佳；含氟固体（D、E、F组）比含氟液体（c组）抑制牙釉质脱矿作用效果更好。Objective To evaluate casein phosphopeptide-amorphous calcium phosphate （CPP-ACP） and several fluoride products in inhibiting enamel demineralization. Methods Seventy healthy premolars extracted before orthodontic treatment were divided into seven groups （A： CPP-ACP; B： CPP-ACP ＋ fluoride mouth rinses; C： fluoride mouth rinses; D： fluoride glass ionomer protection; E： fluoride resin binder; F： fluoride varnish; G：control group）. There were ten samples in each group. The teeth were dipped into an artificial caries solution ten minutes at a time, then applied separately with CPP-ACP, CPP-ACP ＋ fluoride mouth rinses, fluoride mouth rinses, fluoride glass ionomer protection, fluoride resin binder and fluoride varnish on labial enamel. The samples were dipped into an artificial saliva solution （ 37 ℃ ）. Then all samples were examined with atomic force microscope （AFM） at the end of first, second and third monthes. Results At the end of the first month, surface roughness in group D [ （ 114 ± 1） nm] was significantly lower than that in group G[ （172 ±9） nm]. At the end of the second month and the third month, significant difference was found in surface roughness between group G and the rest of groups （ P 〈 0. 05 ） ; between group B and the rest of groups; and between group C and groups （ D, E and F）. Conclusions CPP-ACP and fluoride could both inhibit enamel demineralization in vitro. Solid fluoride （groups D, E and F） had better results than liquid fluoride （group C）.

关 键 词：磷酸钙类 酪蛋白类 牙釉质 牙再矿化 

分 类 号：R783.5[医药卫生—口腔医学]