ZnPcH1介导的光动力疗法净化白血病SHI-1细胞  

Leukemia SH-1 Cells Purged by ZnPcH_1-Based Photodynamic Therapy

作　　者：林晓岚[1,2] 黄慧芳[2] 陈万紫[1,2] 

机构地区：[1]福建医科大学附属协和医院检验科,福建福州350001 [2]福建医科大学附属协和医院血液病研究所,福建省血液病学重点实验室,福建福州350001

出　　处：《中国实验血液学杂志》2012年第4期842-846,共5页Journal of Experimental Hematology

基　　金：福建省自然基金(编号2010J01166);省高等学校新世纪优秀人才支持计划(编号NCETFJ0711);福建省血液病学重点实验室经费(编号2009J1004)

摘　　要：本研究探讨新型酞菁类光敏剂二磺基二邻苯二甲酰亚胺甲基酞菁锌(ZnPcH1)介导的光动力疗法(PDT)对急性单核细胞白血病SHI-1细胞的杀伤作用及其机制,为PDT应用于白血病自体骨髓体外净化提供理论依据。以SHI-1细胞作为研究对象,应用MTT比色法检测细胞增殖。采用AO/EB复合染色、TUNEL、DNA二倍体分析、An-nexin-Ⅴ-FITC/PI双染法等分析细胞的死亡方式。SHI-1细胞与正常骨髓单个核细胞(MNC)以1∶100-1∶10 000混合建立混合细胞模型,通过巢式RT-PCR检测SHI-1细胞融合基因MLL/AF6 mRNA的表达以研究ZnPcH1-PDT对骨髓MNC中所掺入的SHI-1细胞的净化效果。结果表明,ZnPcH1-PDT对SHI-1细胞有杀伤作用,且呈量效关系。Zn-PcH1-PDT能诱导细胞凋亡,且呈时间依赖性。0.5μmol/L ZnPcH1-PDT可完全杀灭模拟缓解骨髓中掺入的SHI-1细胞。结论:ZnPcH1-PDT有希望成为新的高效而简便的骨髓净化手段。The objective of this study was to investigate the effect of a novel Zinc phthalocyanine （ ZnPcH1 ） based photodynamic therapy （PDT） on acute monocytic leukemia cell lines SHI-1 and its mechanism, so as to provide theory basis for bone marrow purging in virto for patients with leukemia. The killing effect of ZnPcH1 -PDT on SHI-1 cells were assessed by MTT method; the SHI-1 cell death patterns were analyzed by AO/EB fluorescence staining, TdT-mediated dUTP nick end labeling （TUNEL）, DNA ploidy analysis, and Annexin V-FITC/PI double staining. Cell mixture was established by integrating SHI-1 cells with normal bone marrow MNC （ by 1 ： 100 - 1 ： 10 000 ）. Purging effect of ZnPcH1-PDT against SHI-1 mixed into normal MNC was assessed by analyzing the expression of fusion gene MLL/AF6 mRNA using nested RT-PCR. The results showed that ZnPcH1-PDT could effectively inhibit SHI-1 cell proliferation in dose-dependent manner, and ZnPcH1 -PDT could induce cell apoptosis in time-dependent manner. 0.5 μ mol/L ZnPcH1- PDT could completely photoinactivated kill SHI-1 cells in the simulated remission bone marrow. It concluded that ZnPcH1-PDT may be a effective and convenient promising purging technique for leukemia.

关 键 词：酞菁类光敏剂 光动力疗法 白血病 SHI-1细胞 骨髓净化 

分 类 号：R733.7[医药卫生—肿瘤] R454.2[医药卫生—临床医学]