蝎毒多肽提取物对K562细胞PI3K和p-Akt信号蛋白表达及细胞增殖的影响  

Influence of Polypeptide Extract from Scorpion Venom on PI3K and p-Akt Signaling Protein Expression and Cell Proliferation of K562 Cells

作　　者：于文俊[1] 杨文华[2] 杨向东[2] 史哲新[2] 王兴丽[2] 郝征[1] 张佳[1] 

机构地区：[1]天津中医药大学,天津300193 [2]天津中医药大学第一附属医院,天津300193

出　　处：《中国实验血液学杂志》2012年第4期872-875,共4页Journal of Experimental Hematology

基　　金：教育部博士点基金;编号20091210110004

摘　　要：本研究旨在探讨蝎毒多肽提取物(PESV)对人慢性髓系白血病细胞系K562细胞凋亡调控的PI3K和p-Akt信号蛋白的影响及作用机制。将体外培养的K562细胞,经PESV处理不同时间后,通过MTT法检测细胞增殖曲线,Western blot法检测PI3K及p-Akt蛋白水平变化。结果表明,与对照组相比,PESV处理后K562细胞凋亡率增加,PI3K及p-Akt蛋白表达降低。结论:PESV可能通过抑制凋亡调控的PI3K、p-Akt信号蛋白,抑制K562细胞增殖,促进细胞凋亡。This study was aimed to investigate the effect of polypeptide extract from scorpion venom （PESV） on PI3K, p-Akt signal protein regulating K562 cell apoptosis and its mechanism. The K562 cells were cultured with PESV for different time, the cell growth curve was determined by MTT method, the levels of PBK and p-Akt proteins were detected by Western blot. The results showed that as compared with control group, the apoptosis rate of K562 cells treated with PESV increased, the levels of PI3K and p-Akt expression decreased. It is concluded that the PESV inhibits the proliferation and promotes the apoptosis of K562 cells probably through suppressing the expression of PI3K and p-Akt signal proteins.

关 键 词：蝎毒多肽提取物 K562细胞 凋亡 PI3K蛋白 p—Akt蛋白 

分 类 号：R733.72[医药卫生—肿瘤] R979.1[医药卫生—临床医学]