DAPT对骨髓瘤细胞RPMI8226增殖和凋亡的影响  

Effect of DAPT on Proliferation and Apoptosis of Human Multiple Myeloma Cell Line RPMI8226

作　　者：原莹莹 曾志勇[2] 陈君敏[2] 

机构地区：[1]福建医科大学第一临床医学院 [2]福建医科大学附属第一医院血液风湿科,福建福州350005

出　　处：《中国实验血液学杂志》2012年第4期922-925,共4页Journal of Experimental Hematology

基　　金：国家自然科学基金(编号30871111);福建省自然科学基金(编号2011J05064)

摘　　要：本研究旨在探讨DAPT对人多发性骨髓瘤细胞系RPMI8226体外增殖的抑制作用及相关机制。用CCK-8法检测RPMI8226细胞的增殖率,用流式细胞术检测凋亡率,用Western blot法检测RPMI8226细胞Notchl、Hes1蛋白表达情况。结果表明,DAPT 0.5-5.0μmol/L作用于RPMI8226细胞24-72 h后,细胞增殖水平明显下降,呈时间和浓度依赖性(r=1.000)。不同浓度的DAPT能诱导RPMI8226细胞发生凋亡,与对照组相比差异有统计学显著性;随着DAPT浓度的增加,Notch1、Hes1蛋白的表达逐渐下调(rNotch=-0.979,rHes1=-0.988)。结论:DAPT能够抑制RPMI8226细胞的增殖,其机制可能与RPMI8226细胞中Notch1、Hes1蛋白的下调有关。The aim of this study was to explore the effect of DAPT （N-[ N-（ 3,5-difluorophenacetyl ）-L-alanyl ]-S- phenylglycinet-butyl ester） on proliferation in vitro of human multiple myeloma cell line RPMI8226 and its underlying mechanism. The proliferation of RPMI8226 cells was detected by CCK-8 method; flow cytometry was employed to assay the cell apoptosis rate;the expressions of Notchl and Hesl proteins were detected by Western blot. The results indicated that the proliferation of human RPMI8226 cells significantly decreased after treatment with DAPT 0.5 - 5.0 μ mol/L for 24 - 72 h （ P 〈 0.05 ） in a concentration- and time-dependent manner. DAPT significantly induced apoptosis of RPMI8226 cells （ P 〈 0.05 ）. The expressions of Notchl and Hesl proteins were gradually down,regulated with the increase of DAPT concentration. It is concluded that the DAPT can inhibit the proliferation of RPMI8226 cells, which may be related with the down-regulation of the protein expression of Notchl and Hesl.

关 键 词：多发性骨髓瘤 RPMI8226细胞 DAPT NOTCH信号通路 

分 类 号：R733.3[医药卫生—肿瘤] R979.1[医药卫生—临床医学]