组织因子微颗粒的检测及其在出凝血异常中的临床意义  

Detection of Tissue Factor-Positive Microparticles and Its Clinical Significance in the Haemostatic Disorder

作　　者：周莉莉[1] 韩悦[1] 朱倩[1] 胡璐萍[1] 赵世香[1] 朱明清[1] 戴兰[1] 沈文红[1] 陈黎[1] 吴德沛[1] 

机构地区：[1]苏州大学附属第一医院血液科,江苏省血液研究所,卫生部血栓与止血重点实验室,江苏苏州215006

出　　处：《中国实验血液学杂志》2012年第4期937-940,共4页Journal of Experimental Hematology

基　　金：江苏省高校自然科学基金(编号09KJB320014);江苏省人事厅六大高峰人才项目(第五批)资助

摘　　要：本研究应用流式细胞术(FCM)建立组织因子微颗粒的检测方法,并探讨其在出凝血异常中的临床意义。应用特异性荧光抗体CD142-PE标记组织因子,采用FCM建立组织因子微颗粒的检测方法。测定20例血栓性疾病患者及25例初治急性早幼粒细胞白血病(APL)患者治疗前后的组织因子微颗粒,观察组织因子微颗粒在各组患者中的变化。结果表明,20例血栓性疾病患者组织因子微颗粒〔(123.28±197.03)/μl〕明显高于20例健康对照组〔(33.27±16.14)/μl,P<0.05〕;25例APL患者治疗前组织因子微颗粒为(75.24±104.58)/μl,亦高于正常对照组(P<0.05),经过诱导治疗缓解后组织因子微颗粒水平明显下降至(34.24±25.20)/μl(P<0.01)。在这25例APL患者中,18例合并弥散性血管内凝血(DIC)患者的组织因子微颗粒在治疗前后有明显差异(P<0.05),7例未合并DIC患者治疗前后的组织因子微颗粒水平未见明显变化。结论:采用流式细胞术检测组织因子微颗粒可对血栓性疾病的诊断提供帮助,并对APL的DIC状况与疾病预后做出评估。Objective of this study was to detect the level of tissue factor-positive microparticles （ TF ^＋ MP） by flow cytometry （FCM） and to analyze its clinical significance in the haemostatic disorder. TF＋ NIP was detected by FCM using antibody CD142-PE in 25 cases of acute promyelocytic leukemia （APL）, 20 cases of hemostatic diseases and 20 healthy adults as controls. The differences of TF^＋ MP between various groups were determined. The results showed that the level of TF^＋ MP in the patients with thrombotic complications was significantly higher than that in the healthy adults （ P 〈 0.05 ）. The TF^＋ MP level was higher in the patient with APL than that in the healthy adults, especially in course before therapy （P 〈 0.01 ）, but the difference was not statistically significant in the patient with APL after therapy and the healthy adults. Among these patient with APL, the level of TF^＋ MP in the 18 patients who complicated with dissem- inated intravascular coagulation （DIC） was also higher than that in the healthy adults （ P 〈 0.05 ）, but the level of TF^＋ MP in the other 7 patients who did not complicate with DIC was similar before and after treatment. It is concluded that the method of TF^＋ MP detection by FCM is feasible and simple, it is useful for the diagnosis of thrombotic disorder, and helps evaluation for the prognosis of APL patient.

关 键 词：组织因子微颗粒 急性早幼粒细胞白血病 血栓性疾病 流式细胞技术 

分 类 号：R365.15[医药卫生—病理学] R733.71[医药卫生—基础医学]