UV-B辐射下悬沙对小球藻生长和DNA损伤的影响  

Effects of Suspended Solids on Growth and DNA Damage of Chlorella sp. Exposed to UV-B Radiation

作　　者：李云[1] 黄耀佳[2] 王晓蓉[2] 

机构地区：[1]中国水产科学研究院东海水产研究所,上海200090 [2]华东师范大学生命科学学院,上海200062

出　　处：《应用与环境生物学报》2012年第4期605-609,共5页Chinese Journal of Applied and Environmental Biology

基　　金：国家重点基础研究发展规划项目(No.2010CB429005);中央级公益性科研院所基本科研业务费专项资金(中国水产科学研究院东海水产研究所)项目(No.2008M13)资助~~

摘　　要：研究了UV-B辐射(17μW cm-2,5 min/d)下不同浓度(0、100和1 000 mg L-1)和粒径组成(全粒径组和粒径<38μm组)的悬沙对小球藻(Chlorella sp.)生长和DNA损伤的影响.结果显示,小球藻的生长可用Logistic增长模型拟合,拟合后的生长参数表明悬沙的"遮荫效应"减轻了UV-B辐射对小球藻细胞的DNA损伤,从而对小球藻生长产生正影响.悬沙浓度越高,UV-B辐射对小球藻细胞DNA损伤程度越低,小球藻的环境负载能力a和瞬时增长率K越大.当悬沙浓度为100 mg L-1时,全粒径组a值和K值小于粒径<38μm组,且两组间小球藻细胞DNA损伤差异显著(P<0.05),但当悬沙浓度达1 000 mg L-1时,全粒径组a值和K值大于粒径<38μm组,两组间细胞的DNA损伤无显著差异(P>0.05).Effects of suspended solids （SS） with different concentrations （0, 100 and 1 000 mg L-1） and different particle sizes （all size range and size 〈 38 ？m） on the growth and DNA damage of Chlorella sp. exposed to UV-B radiation （17 ？W cm-2, 5 min/d） were studied. The growth of Chlorella sp. was well predicted by a logistic model, and the growth parameters showed that, SS attenuated DNA damage induced by UV-B and promoted the cell growth. With the increase of SS concentration, the cellular DNA damage induced by UV-B decreased, and the environmental carry capacity （a） and instantaneous rate of increase （K） increased. When the cells were exposed to SS of 100 mg L-1, the values of a and K were lower in all-size-range group than in the group of size 〈 38 ？m, and a significant difference in the cellular DNA damage （P 〈 0.05） appeared between them. When exposed to SS of 1 000 mg L-1, a and K were higher in all-size-range group than in the group of size 〈 38 ？m, but no significant difference in the cellular DNA damage （P 〉 0.05） was found. Fig 3, Tab 2, Ref 24

关 键 词：悬沙 UV-B辐射 小球藻 细胞密度 DNA损伤 

分 类 号：X173[环境科学与工程—环境科学]