RNA干扰沉默MTA1基因对膀胱癌细胞失巢凋亡的影响  

Studies of siRNA MTA1 on anoikis of human bladder cancer cell line BIU-87

作　　者：崔飞伦[1,2] 邱镇[1] 胡建鹏[1] 范钰[2] 

机构地区：[1]江苏大学附属人民医院泌尿外科,江苏镇江212002 [2]江苏大学附属人民医院肿瘤研究所,江苏镇江212002

出　　处：《江苏大学学报（医学版）》2012年第4期324-327,共4页Journal of Jiangsu University：Medicine Edition

基　　金：镇江市科技计划项目(SH2008035)

摘　　要：目的:观察RNA干扰沉默MTA1基因对膀胱癌细胞株BIU-87增殖和失巢凋亡的影响。方法:构建针对MTA1基因的小干扰RNA(small interfering RNA,siRNA),应用MTA1 siRNA转染处理人膀胱癌细胞株BIU-87后,分别采用实时定量PCR和蛋白质印迹法检测MTA1基因mRNA和蛋白的表达,采用软琼脂集落培养试验检测锚着不依赖性增殖,琼脂糖凝胶电泳和流式细胞术检测BIU-87细胞失巢凋亡。结果:与对照组比较,MTA1 siRNA转染组MTA1 mRNA和蛋白水平明显下降,且呈浓度依赖性(P<0.01)。MTA1 siRNA转染组软琼脂集落形成数明显减少,且与浓度相关(P<0.01)。琼脂糖凝胶电泳和流式细胞术结果显示,MTA1 siRNA转染可诱导BIU-87细胞失巢凋亡,且与浓度相关(P<0.01)。结论:MTA1 siRNA可抑制膀胱癌细胞株BIU-87增殖,诱导失巢凋亡可能是其机制之一。Objective： To observe the effects of MTA1 small interfering RNA（siRNA）on anchorage-independent growth and anoikis of bladder cancer cell line BIU-87.Methods： After construction of the MTA1 gene eukaryotic expression vector of siRNA and transfected to the bladder cancer cell line BIU-87,the expression of MTA1 gene was detected by real-time PCR and western blot;to detect anchorage-independent growth using clon formation in soft agar,and anoikis using DNA fragmentation assay and flow cytometry assay,respectively.Results： The level of mRNA and protein of MTA1 of groups transfectd with siRNA were decreased markedly in a time-and dose-dependent manner.MTA1 siRNA can inhibit anchorage-independent growth in a dose-dependent manner.MTA1 can reduced the resistance to anoikis of BIU-87 cells in a dose-and time-dependent manner.Conclusion： MTA1 siRNA can inhibit the growth of bladder cancer cell line BIU-87 through inducing anoikis.

关 键 词：膀胱肿瘤 转移相关基因1 失巢凋亡 

分 类 号：R737.14[医药卫生—肿瘤]