RP-HPLC法测定金果饮咽喉片中没食子酸的含量  

Determination of Gallic acid in Jinguoyin Yanhou tablets by RP-HPLC

作　　者：秦贻强[1] 蔡小玲[1] 董晓敏[1] 

机构地区：[1]广西桂林医学院附属医院,桂林541001

出　　处：《海峡药学》2012年第7期53-55,共3页Strait Pharmaceutical Journal

摘　　要：目的建立金果饮咽喉片中没食子酸的含量测定方法。方法采用RP-HPLC测定,色谱柱为Sino Chrom ODS-APC18（250mm×4.6mm,5μm）,流动相为甲醇-水-磷酸（6∶94∶0.2）,流速1.0mL·min-1,检测波长266nm。结果没食子酸在6-36μg·mL-1浓度范围内呈良好的线性关系（r=0.9996）,平均回收率为99.35%,RSD=0.85%。结论本方法准确、简便、重现性好,可作为金果饮咽喉片中没食子酸的含量测定方法。OBJECTIVE To establish a gallic acid content determination method in Jinguoyin Yanhou Tablet. METHODS The chromatographic column is Sino Chrom C4s column. The mobile phase is methanol-water-phosphate （6：94：0.2）.The wavelength detection is 266 nm.The flow rate is 1.0mL. rain-t .RESULTS The linear range of gallic acid was 6μg.mL-1-36μg. mL-1 （r = 0. 9996）. The average recovery is 99.35 %, RSD = 0.85 %. CONCLUSION The method is simple, rapid, accurate, and with good reproducibility for the determination of gallic acid in Jinguoyin Yanhou Tablet.

关 键 词：金果饮咽喉片 没食子酸 RP-HPLC 含量测定 

分 类 号：R927.2[医药卫生—药学]