藏紫草化学成分及抑菌活性研究  

Study on Chemical Constituents and Antibacterial Activity of RADIX ONOSMATIS

作　　者：黄艳[1] 谭睿[1] 王静[1] 罗琴[1] 崔文婷[1] 吴梅[1] 曹雨虹[1] 

机构地区：[1]西南交通大学生命科学与工程学院,四川成都610031

出　　处：《安徽农业科学》2012年第23期11553-11554,共2页Journal of Anhui Agricultural Sciences

摘　　要：[目的]研究藏紫草的化学成分及其抗菌活性。[方法]采用浓度95%乙醇冷浸提取藏紫草得到总浸膏(A),依次采用不同溶剂进行萃取,得到石油醚提取物(B)、乙酸乙酯提取物(C)和正丁醇提取物(D),将C通过硅胶柱色谱技术分离纯化,并采用光谱学方法鉴定化合物的结构;用滤纸片法对A、B、C和D进行抑菌活性研究,并用2倍稀释法测定其MIC值。[结果]从C中分离得到5个化合物,分别为β-谷甾醇、齐墩果酸、苯甲酸、阿魏酸和胡萝卜苷。[结论]化合物1～5为首次从该植物中分离得到;A、B、C和D均具有一定的抗菌活性。[Objective] To study the antibacterial activity of chemical constituents in RADIX ONOSMATIS.[Method] The chemical constituents were firstly extracted by 95% ethanol water to get ethanol extract（A）.After that it was extracted to get petroleum ether extract（B）,ethyl acetate extract（C）,and n-butanol extract（D）,C was isolated and purified by column chromatography,and their structures were identified by spectroscopic analysis.The antifungal activities of A,B,C and D were studied by K-B method,and the MIC was detected with two-times dilution method.[Result] Five compounds were isolated and identified as β-sitosterol,oleanolic acid,benzoic acid,ferulic acid and daucosterol.[Conclusion] All the five compounds were isolated from this plant for the first time.A,B,C and D showed good antibacterial activity.

关 键 词：藏紫草(RADIX ONOSMATIS) 化学成分 抑菌活性 

分 类 号：S567[农业科学—中草药栽培]