趋化因子CXCL11及其受体CXCR3在重症急性胰腺炎相关肺损害中的作用  

Expression of CXCL11 and CXCR3 in acute lung injury associated with severe acute pancreatitis

作　　者：吴兴[1] 张海峰[2] 丁晓凌[2] 强晖[2] 曹维[2] 周国雄[2] 

机构地区：[1]泗洪县人民医院消化科,江苏省宿迁市223900 [2]南通大学附属医院消化内科,江苏省南通市226001

出　　处：《世界华人消化杂志》2012年第21期1967-1972,共6页World Chinese Journal of Digestology

基　　金：南通市社会发展基金资助项目;No.S5054~~

摘　　要：目的:制作大鼠重症急性胰腺炎(severe acute pancreatitis,SAP)模型,检测不同时间点趋化因子CXCL11及其受体CXCR3在SAP肺组织中的动态变化,探讨他们在SAP肺功能损害过程中的作用.方法:48只SD大鼠,雌雄不限,随机分为2组:对照组(C组),SAP组(P组),每组24只.4%牛黄胆酸钠逆行胰胆管注射建立SAP大鼠模型,剂量为1mL/kg,C组打开腹腔后仅仅翻动胰腺组织数次.每组随机分为4个亚组,每个亚组6只.4个组分别在1、3、6、12h抽血、处死,留取组织标本.分别检测各不同时间点组的血清淀粉酶、肺湿干重比,胰腺组织、肺组织病理,免疫组织化学法检测肺CXCL11及CXCR3的表达,酶联免疫吸附试验(ELISA)检测血清中的CXCL11的水平.结果:P组各亚组血清淀粉酶值明显升高(P<0.01vsC组);肺湿干重比值:P组3、6、12h组较C组明显升高(P<0.05);胰腺组织、肺组织病理:3、6、12hP组肺组织损伤明显;免疫组织化学显示P组CXCL11与CXCR3蛋白表达较C组表达明显增强(P<0.05),ELISA显示:1、3、6、12hP组血清CXCL11蛋白较C组明显增高(P<0.01).结论:CXCL11/CXCR3可能参与大鼠SAP急性肺功能损害的发病过程.AIM：To investigate the expression of chemokine CXCL11 and its receptor CXCR3 in acute lung injury associated with severe acute pancreatitis（SAP）.METHODS：Forty-eight SD rats were randomly and equally divided into two groups：control group and SAP group.SAP was induced in rats of the SAP group by retrograde injection of 4% sodium taurocholate into the bili-pancreatic duct.Each group was further randomly and equally divided into four subgroups for testing at different time points.The rats were randomly selected to be executed at 1,3,6,and 12 h after induction of SAP.Serum amylase,wet/dry weight ratio of the lung,and histological changes of the lung were measured.Expression of CXCL11 and CXCR3 proteins in the lung was detected by immunohistochemistry.Serum levels of CXCL11 were measured by enzyme-linked immunosorbent assay（ELISA）.RESULTS：Serum amylase was significantly higher in the SAP group than in the control group（P 〈 0.01）.The lung wet/dry weight ratio was significantly higher at 3,6,and 12 h in the SAP group than in the control group（all P 〈 0.05）.The expression of CXCL11 and CXCR3 in the lung（all P 〈 0.05） and serum levels of CXCL11 at various time points（all P 〈 0.01） were significantly higher in the SAP group than in the control group（all P 〈 0.05）.CONCLUSION：CXCL11 and CXCR3 may play important roles in the pathogenesis of acute lung injury in SAP.

关 键 词：重症急性胰腺炎 CXCL11 CXCR3 急性肺损伤 

分 类 号：R576[医药卫生—消化系统]