NF-κB在鼠肝纤维化组织中的表达及其与α-SMA、Ⅲ型胶原的相关性  

Correlation of nuclear factor κB expression with α-SMA and collagen Ⅲ expression in hepatic fibrosis in rats

作　　者：朱净[1] 范建荣[1] 潘亮[1] 黄华[2] 肖明兵[1] 江枫[1] 陆翠华[1] 

机构地区：[1]南通大学附属医院消化内科,江苏省南通市226001 [2]南通大学附属医院病理科,江苏省南通市226001

出　　处：《世界华人消化杂志》2012年第22期2081-2085,共5页World Chinese Journal of Digestology

摘　　要：目的:研究核转录因子B(Nuclear factor kappaB,N F-B)在鼠肝纤维化组织中的表达及其与-平滑肌肌动蛋白(-smooth muscle actin,-SMA)、Ⅲ型胶原相关性.方法:32只♂清洁级SD大鼠随机分为正常对照组8只和模型组24只,用CCl4皮下注射法制备肝纤维化模型组,免疫组化和RT-PCR方法联合检测NF-B、-SMA、Ⅲ型胶原蛋白及基因在肝纤维化组织中的动态表达,并对3者表达作相关性分析.结果:正常对照组肝组织中NF-B、-SMA及Ⅲ型胶原的蛋白及基因均有微量表达;CCl4注射后2、4和6 wk肝组织NF-B、-SMA及Ⅲ型胶原的蛋白及基因表达呈明显递增趋势(P<0.05),2 wk后3者表达呈显著正相关(P<0.05).结论:NF-B表达随着肝纤维化程度的加重而增强,在肝纤维化的发生和发展中起重要作用.AIM： To investigate the correlation of expression of nuclear factor κB with that of-smooth muscle actin（α-SMA） and collagen Ⅲ in hepatic fibrosis in rats.METHODS： Thirty-two male SD rats of SPF grade were divided randomly into control group and model group.Hepatic fibrosis was induced in rats by injecting carbon tetrachloride.The mRNA and protein expression of NF-κB,-SMA,and collagen Ⅲ was examined using reverse transcription-polymerase chain reaction（RT-PCR） and immunohistochemistry,respectively.The correlation of NF-κB expression with-SMA and collagen Ⅲ expression was then analyzed.RESULTS： NF-κB,α-SMA and collagen Ⅲ were lowly expressed in normal liver tissue.After injection of carbon tetrachloride,the expression of NF κB,-SMA and collagen Ⅲ mRNAs and proteins began to increase at week 2 and was significantly higher at weeks 4 and 6（both P 〈 0.05）,showing a gradually rising trend.There was a positive correlation between the expression of NF-κB and that of-SMA and collagen Ⅲ（both P 〈 0.05）.CONCLUSION： NF-κB plays an important role in the pathogenesis of hepatic fibrosis.

关 键 词：肝纤维化 核转录因子ΚB Α-平滑肌肌动蛋白 Ⅲ型胶原蛋白 

分 类 号：R575.2[医药卫生—消化系统]