不同分化程度的大肠癌患者肠黏膜血管紧张素Ⅱ及其Ⅰ型受体的表达差异与意义  

Expression of Angiotensin Ⅱ and AT_1 R in colorectal cancer

作　　者：杨胜兰[1] 沈霖[1] 高洁嫦[1] 樊琼[1] 黄璐[1] 吴嫣然[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院中西医结合科,湖北省武汉市430022

出　　处：《世界华人消化杂志》2012年第23期2213-2217,共5页World Chinese Journal of Digestology

基　　金：国家自然科学基金资助项目;No.30873225~~

摘　　要：目的:比较分化程度不同的大肠癌患者与无器质性肠道疾病志愿者肠黏膜血管紧张素Ⅱ(AngiotensinⅡ)及其Ⅰ型受体(AngiotensinⅡtypeⅠreceptor,AT1R)表达差异.方法:肠镜室募集大肠癌患者24例(病理学检查证实其中9例为大肠高分化腺癌患者,9例为大肠中分化腺癌患者,6例为大肠低分化腺癌患者)及6例无器质性肠道疾病志愿者,电子结肠镜下取活检获得新鲜的肠黏膜组织.免疫组织化学法检测各组肠黏膜AngiotensinⅡ及AT1R蛋白表达强度,单因素方差分析各组之间的差异.结果:大肠腺癌(低、中、高分化组)患者肠黏膜AngiotensinⅡ和AT1R的表达较无器质性肠道疾病志愿者肠组织AngiotensinⅡ和AT1R表达明显增强(21.24±4.60,19.90±5.16,17.87±5.30vs 2.45±0.83;15.98±3.66,8.13±2.49,8.12±2.13 vs 2.53±0.82,均P<0.01);不同分化程度的各组之间AngiotensinⅡ表达未见明显差异(P>0.05);分化程度越高AT1R表达越少(P<0.01).结论:AngiotensinⅡ及其Ⅰ型受体的表达与大肠癌存在一定的关系,其深入机制需要进一步通过动物实验及体外实验来证实.AIM： To investigate the expression of Angiotensin Ⅱand AT1R in colorectal cancer.METHODS： Fresh colonic specimens were obtained from 6 healthy volunteers and 24 patients suffering from colorectal cancer（including 6 patients with poorly differentiated adenocarcinoma,9 patients with moderately differentiated adenocarcinoma and 9 patients with well differentiated adenocarcinoma） by endoscopic punch biopsy.The diagnosis was confirmed pathologically.Immunohistochemistry was used to examine the expression of Angiotensin Ⅱand AT 1 R in the colon of patients with colorectal cancer and healthy volunteers.RESULTS： The expression of Angiotensin Ⅱ and AT 1 R proteins in the colon of patients with colorectal cancer with different differentiation grades was significantly higher than that in normal controls（21.24 ± 4.60,19.90 ± 5.16,17.87 ± 5.30 vs 2.45 ± 0.83;15.98 ± 3.66,8.13 ± 2.49,8.12 ± 2.13 vs 2.53 ± 0.82,all P 0.05）.Compared to moderately and well differentiated adenocarcinoma,the expression of AT 1 R was higher in poorly differentiated grade（both P 〈 0.01）.CONCLUSION： These data suggest that Angiotensin Ⅱ and AT 1 R perhaps play an important role in the pathogenesis of colorectal cancer.

关 键 词：大肠癌 血管紧张素Ⅱ 血管紧张素Ⅱ及其Ⅰ型受体 信号通路 

分 类 号：R735.34[医药卫生—肿瘤]