苦菜中总黄酮的提取与含量测定  

Extraction and Content Determination of Flavonoids in Sonchus oleraceus L

作　　者：楚红英[1] 李瑜 李华北[1] 袁琦[3] 

机构地区：[1]黄河水利职业技术学院环境与化学工程系,河南开封475004 [2]开封市中医院泌尿外科,河南开封475004 [3]河南大学药学院,河南开封475004

出　　处：《安徽农业科学》2012年第24期12017-12019,共3页Journal of Anhui Agricultural Sciences

基　　金：河南省教育厅科学技术研究重点项目(12B150016)

摘　　要：[目的]研究苦菜中总黄酮的提取方法和总黄酮含量随生长月份、部位的变化关系。[方法]采用正交试验设计提取总黄酮的最佳试验方案。[结果]所选取的3个提取因素的主次关系为,提取溶剂浓度>料液比>提取时间,最佳提取条件为:乙醇浓度75%,料液比1∶20(g/ml,W/V,下同),提取时间为2 h。苦菜中不同部位总黄酮含量的变化关系为花>叶>茎>根;4月份苦菜的总黄酮含量最高。[结论]试验为合理开发利用苦菜资源提供了一定的理论基础。[ Objective ] To study the extraction methods of flavonoids from Sonchus oleraceus L and the variations of their contents with growth months and parts. [ Method] The contents of flavonoids in S. oleraceus grown at different months and their distributions at different parts of S. oleraceus were detailed studied in this article. The extraction process of total flavonoids from S. oleraeeus leaves were optimized through orthog- onal design method. [ Result] It was discerned from the experiment that the extraction solvent concentration 〉 solid-liquid ratio 〉 extraction time. The optimized extraction condition was that the extractant to raw material is 1：20 g/ml, extraction solvent was 75% alcohol and extrac- tion time was 2 h. The flavonoids contents in different parts were flower 〉 leaf 〉 stem 〉 root. Flavonoids in S. oleraceus L collected in April were higher than that of other months. [ Conclusion] The result was valuable for further development and utilization of S. oleraceus resources.

关 键 词：苦菜(Sonchus oleraceus L) 黄酮类化合物 提取 

分 类 号：S567.2[农业科学—中草药栽培]