晶状体自发肿瘤转基因鼠脑横截面标本制备及鉴定  

The Preparation and Identification of Head Longitudinal Specimen of Transgenic Mice with Spontaneous Lens Tumor

作　　者：王建平[1] 张梅英[2] 勾文峰[1] 高野康雄 郑华川[1] 

机构地区：[1]中国医科大学基础医学院生物化学与分子生物学研究室,病理与病理生理研究所,沈阳110001 [2]中国医科大学实验动物中心,沈阳110001 [3]日本神奈川县立癌中心临床研究所,日本横滨241-0815

出　　处：《中国医科大学学报》2012年第8期673-675,共3页Journal of China Medical University

基　　金：国家自然科学基金资助项目(81172371);教育部归国人员科研启动基金(2009-03);人事部留学人员科技活动择优资助项目(2008-01);沈阳人才资源开发基金;沈阳市科技攻关项目

摘　　要：目的确定JCV T抗原诱发晶状体肿瘤转基因鼠脑中肿瘤来源及路径。方法 18月龄转基因鼠麻醉后4%多聚甲醛灌流固定,EDTA脱钙颅骨软化后脑经横截制备石蜡标本并进行HE染色。免疫组化和免疫荧光确定脑组织T抗原表达。结果经过脱钙处理后颅骨组织非常软,横截后发现白色肿瘤组织蔓延至脑组织中,HE染色确定眼部和脑部白色组织均为晶状体肿瘤细胞,免疫组化和免疫荧光结果显示细胞核中存在T抗原强阳性表达。结论 JCV T抗原诱发晶状体肿瘤是经过视神经孔进入脑组织,鼠头横截面组织标本制备对研究头部肿瘤的毗邻关系和肿瘤组织在脑组织中侵袭部位极具应用价值。Objective To clarify the source and pathway of brain tumor in transgenic mouse with JCV T antigen-induced spontaneous lens tumors. Methods 18-month-old transgenic mouse was fixed by neutral 4% paraformaldehyde perfusion during anesthesia. After decalcified with EDTA, the softened skull was longitudinally resected and subjected to the routine preparation of paraffin-embedded block and subsequent hem_atoxylin-eosin staining. T antigen was detected in the lens lesions by immunohistochemistry and immunofluorescence. Results The skull became softer after decalcification and longitudinal observation showed white tumor tissue spread firm eye to brain. HE staining indicated that there were many lens tumor cells in the part of white tissue. Immunohisotchemical staining and immunofluorescence displayed the positivity of T antigen in the nuclei of lens tumor cells. Conclusion The lens tumor could enter the brain by foremen opticum, h is of great significance to evaluate tumor invasion and invasive parts in brain in preparing of the head longitudinal specimen of transgenic mice with spontaneous lens tumor.

关 键 词：转基因鼠 晶状体自发肿瘤 头横截面 免疫组化 T抗原 

分 类 号：R739.7[医药卫生—肿瘤]