银杏苦内酯B对内毒素血症幼年大鼠肠氧化损伤的保护作用  

The Protective Effect of Ginkgolide B on Intestinal Oxidative Injury in Young Rats with Endotoxemia

作　　者：王丽杰[1] 孙莹[1] 孙梅[2] 

机构地区：[1]中国医科大学附属盛京医院儿科重症监护病房,沈阳110004 [2]中国医科大学附属盛京医院儿科消化病房,沈阳110004

出　　处：《中国医科大学学报》2012年第8期700-704,共5页Journal of China Medical University

基　　金：辽宁省教育厅高校科研计划(L2010607)

摘　　要：目的氧化应激与炎性反应引起的肠组织损伤密切相关。应用血小板活性因子(PAF)受体拮抗剂银杏苦内酯B对幼年大鼠内毒素血症模型中肠黏膜氧化损伤的保护作用作初步研究。方法 18日龄Wistar大鼠,随机分为内毒素脂多糖(LPS)组和PAF受体拮抗剂组(预防组和治疗组)及对照组,注射LPS后1.5,3,6,24,48,72 h取回肠。LPS组和对照组分别腹腔注射内毒素5 mg/kg及生理盐水1 mL/kg;预防组和治疗组分别于每一时相点注射LPS前、后30 min腹腔注射PAF受体拮抗剂(银杏苦内酯B)BN52021 5 mg/kg。苏木精-伊红染色及透射电镜作形态学检查,剩余部分回肠于-80℃深低温冰箱中保存,检测黄嘌呤氧化酶(XOD)、超氧化物歧化酶(SOD)。结果 LPS组1.5,3,6,24 h可见绒毛水肿,固有层血管充血,间质淋巴管扩张,肠腔炎性渗出,拮抗剂组可见绒毛水肿。电镜下对照组肠微绒毛及细胞间紧密连接未见异常。实验组上皮细胞连接增宽;微绒毛变细、稀疏,部分断裂、脱落;细胞器受损。拮抗剂组改变较实验组减轻。LPS组1.5,3,6,24 h XOD明显高于对照组,6 h从(239.85±17.64)U/g升至(330.99±61.73)U/g(P<0.01),预防组及治疗组XOD变化趋势同LPS组,但各时相点XOD较LPS组略低。LPS组1.5,3,6,24,48 h SOD较对照组明显降低,6 h从(141.55±28.12)U/mg降至(86.89±9.99)U/mg(P<0.01),预防组及治疗组SOD变化趋势同LPS组,但各时相点SOD较LPS组高。结论氧化损伤在内毒素血症肠损伤中发挥一定作用,预防和治疗性应用银杏苦内酯B BN52021可减轻肠道的炎性反应,对肠黏膜损伤具有一定的保护作用。Abstract Objective Oxidative stress is closely correlated with intestinal injury induced by inflammatory response. We investigated the protective effect of platelet activating factor receptor antagonist （Ginkgolide B,BN52021 ） on oxidative injury of intestinal in young rat with endotoxaemia. Methods Eighteen-day-old Wistar rats were randomly divided into 3 groups and treated with hpopolysaccharide （LPS） （ 5 mg/kg）, LPS plus PAF receptor antagonist and normal saline injection （Control） respectively. Ginkgolide B 5 mg/kg was administered before or 30 minutes after LPS injection （pretreatment or treatment）. The ileum specimens （n =8） were harvested at 1.5,3,6,24,48 and 72 h af- ter LPS injection. Hematoxylin and emsin staining and transmission electron microscopy （TEM） was used for histological morphology evalua- tion. The rest samples were stored in -80 ℃ for measurements of xanthine oxidase（XOD） and supemxide dismutase（SOD）. Results His- tologic examination of intestinal injury in LPS group showed edema of the villus, capillary congestion of the lamina propria, extension of the subepithelial, polymorphonuclear infiltration in enteric cavity on 1.5,3,6,24 h. Edemas of the villus were found in antagonist group. Ultrami- crostmctttral change of TEM showed microvilli and tight junctions were intact in the control group. Enlargement of tight junctions were seen in the experiment greup,and the microvilli were thin,rare or disrupt,shed. The rough endoplasmic reticulum,mitochondria,and glycogen parti- cles were injured. The levels of XOD at 1.5,3,6,24 h were obviously higher in LPS group than control group. The content of XOD in- creased mostly at 6 h from （239.85±17.64）U/g to （330.99±61.73）U/g of control group （P 〈 0.01 ）. The tendency of antagonist group was the same as the IPS group. But the levels of XOD of antagonist group were lower than LPS group at each time point. The levels of SOD of 1.5,3,6,24 and 48 h were obviously decreased in LPS group compared with cont

关 键 词：血小板活化因子 超氧化物歧化酶 黄嘌呤氧化酶 胃肠功能障碍 银杏苦内酯B 

分 类 号：R631.1[医药卫生—外科学]