实时定量聚合酶链反应与巢式聚合酶链反应在289例白血病融合基因检测中的比较  

The Comparison of RT-PCR and Nested PCR in Detection of Fusion Genes in 289 Cases with Leukemia

作　　者：王玥[1] 李艳[1] 金晶纯[1] 王亚柱[1] 魏鑫[1] 

机构地区：[1]中国医科大学附属第一医院血液内科,沈阳110001

出　　处：《中国医科大学学报》2012年第8期738-741,共4页Journal of China Medical University

基　　金：辽宁省自然科学基金资助项目(20082117);辽宁省教育厅高校科研计划(2008782)

摘　　要：目的比较实时定量聚合酶链反应(RT-PCR)与巢式聚合酶链反应(nPCR)在白血病融合基因BCR/ABL、PML/RARa及AML1/ETO检测中的结果。方法分别应用RT-PCR与nPCR对289例急慢性白血病初诊和完全缓解的患者BCR/ABL、PML/RARa及AML1/ETO融合基因进行检测,结合患者骨髓细胞形态学及临床转归予以分析。结果 46例初诊慢性粒细胞白血病(CML)患者中,RT-PCR与nPCR检测BCR/ABL融合基因阳性率分别为95.7%和93.5%(P=0.997);69例完全缓解CML患者中,RT-PCR与nPCR检测阳性率分别为78.4%和58.1%(P<0.05)。32例初诊急性早幼粒细胞白血病(APL)患者中,RT-PCR与nPCR检测PML/RARa融合基因阳性率分别为93.8%和90.6%(P=0.996);114例完全缓解APL患者中,RT-PCR与nPCR检测阳性率分别为12.3%和7.0%(P<0.05)。12例初诊急性粒细胞白血病M2(ANLL-M2)患者中,RT-PCR与nPCR检测AML1/ETO融合基因阳性率分别为50.0%和50.0%(P=1.0);16例完全缓解ANLL-M2患者中,RT-PCR与nPCR检测阳性率分别为50.0%和12.5%(P<0.05)。结论 RT-PCR较nPCR更为敏感而准确,应用RT-PCR可以提高微小残留病的检出率,为临床诊断和治疗提供更为有效的分子生物学依据。Objective To explore the differences of real-time quantitative polymerase chain reaction （RT-PCR） and nested polymerase chain reaction （nPCR） in the detection of fusion genes of BCR/ABL,PML/RARa and AML1/ETO in leukemia. Methods RT-PCR and nPCR were applied for detection of the fusion genes BCR/ABL,PML/RARa and AML1/ETO in 289 patients with leukemia,the results com- bined with cytomophology and chnical outcomes of patients were analyzed. Results The positive rates of fusion gene of BCR/ABL detected by RT-PCR and nPCR in 46 cases of newly diagnosed chronic myelocytic leukemia （CML） were 95.7% and 93.5% respectively （P = 0.997） ,while the ratios were 78.4% and 58.1% （P 〈 0.05） in 69 cases of complete remission （CR）. By the same detections,the positive rates of fusion gene of PML/RARa in 32 cases of newly diagnosed acute promyelocytic leukemia （APL） were 93.8% and 90.6% respectively （P =0.996）,while the ratios were 12.3% and 7.0% （P 〈 0.05） in lld cases of CR. The positive rates of fusion gene of AML1/ETO in 12 cases of newly diagnosed acute myeloblastic leukemia M2 （ANLL-M2） were 50.0% and 50.0% respectively （P =1.0）,while the ratios were 50.0% and 12.5% （P 〈 0.05 ） in 16 cases of CR. Conclusion RT-PCR is more sensitive and accurate than nPCR for detecting fusion genes of leukemia. It can improve the detection of minimal residual disease, and provide more effective molecular biological basis for diagnosis and treatment in leukemia.

关 键 词：白血病 融合基因 实时定量聚合酶链反应 巢式聚合酶链反应 

分 类 号：R551.3[医药卫生—血液循环系统疾病]