PPARγ对前列腺癌细胞增殖及糖酵解的影响  

Effects of PPAR-γ on the proliferation and glycolysis metabolism of prostate cancer cells

作　　者：曾祥建[1] 毕学成[2] 戴奇山[3] 韩兆冬[3] 钟惟德[3] 

机构地区：[1]深圳市龙岗中心医院泌尿外科,广东深圳518116 [2]广东省人民医院泌尿外科,广东广州510080 [3]广州市第一人民医院、广东省临床分子医学与分子诊断重点实验室,广东广州510180

出　　处：《中华男科学杂志》2012年第8期692-696,共5页National Journal of Andrology

基　　金：广东省临床分子医学及分子诊断实验室重点实验室项目(2010A060801016);广东省泌尿外科重点实验室项目(2010A060801016)~~

摘　　要：目的:研究PPAR-γ基因表达对前列腺癌细胞增殖和肿瘤特殊糖酵解代谢的影响。方法:采用RNAi技术构建PPARγ低表达的腺病毒载体并转染到前列腺癌细胞株,并以空白载体为对照组,进行细胞增殖检测、细胞凋亡检测、糖酵解代谢基因及产物乳酸检测,并比较两组之间的结果差异。结果:经RNAi抑制的PPAR-γ前列腺癌细胞株与对照组相比,蛋白表达量降低至(26.00±4.06)%,增殖抑制率最大为第2天达(39.50±4.92)%,细胞凋亡率升高至(21.03±3.08)%,糖酵解代谢基因产物(Myc和Glut-1)下降,第4天培养液中乳酸浓度为对照组的69.71%,上述结果具有统计学差异(P<0.01)。结论:抑制PPAR-γ基因的表达有望成为治疗前列腺癌新方法。Objective： To investigate the effects of the expression of the PPAR-γ gene on the proliferation and glycolysis metabolism of prostate cancer cells. Methods ： Using RNAi, we constructed lowly - expressed shRNA-PPAR-γ adenoviruses and transfected them to PC3 prostate cancer cells, with blank vectors as controls. Then we detected the proliferation and apoptosis of the cells, glyeolysis metabolism related genes and lactate accumulation by CCK-8 kit, and compared the results between the two groups. Results： Compared with the control group, the PPAR-γ gene expression was obviously inhibited by RNAi in the PC3 cells, and its protein expression was reduced to （26. 00 士 4.06） %. The proliferation inhibition rate was （39.5 士 4.92） % on the 2nd day, and the apoptosis rate was as high as （21.03 士 3.08）%. The glycolysis metabolism related gene products （Myc and Glut-I ） were significantly decreased, and the lactate concentration was reduced to 69.71% of that of the controls on the 4th day. There were statistically significant differences in the above findings as compared with the control group （P 〈 0. 01 ）. Conclusion： PPAR-γ gene knockdown is expected to be a new way to treat prostate cancer.

关 键 词：前列腺癌 PPARΓ 细胞增殖 糖酵解代谢 

分 类 号：R737.25[医药卫生—肿瘤]