原料血浆及血液制品中人细小病毒B19 DNA的检测  

Determination of human parvovirus B19 DNA in source plasma and blood products

作　　者：侯继锋[1] 王敏[1] 马秋平[1] 

机构地区：[1]中国食品药品检定研究院血液制品室,北京100050

出　　处：《中国生物制品学杂志》2012年第8期1043-1044,1048,共3页Chinese Journal of Biologicals

基　　金：国家科技支撑计划项目(2008BAI54B08)

摘　　要：目的检测我国原料血浆及血液制品中人细小病毒B19(Human parvovirus B19)的污染情况。方法在B19基因组编码区的高度保守区2 000～2 300 bp之间设计PCR引物探针,采用荧光定量PCR法检测单人份血浆、生产用混合血浆、静注人免疫球蛋白、人纤维蛋白原、人凝血因子Ⅷ及人凝血酶原复合物中的B19病毒DNA。结果单人份血浆、生产用混合血浆、静注人免疫球蛋白、人纤维蛋白原、人凝血因子Ⅷ和人凝血酶原复合物的B19病毒DNA阳性率分别为0.092%、82.41%、0、45.83%、67.86%和78.79%。结论我国原料血浆及血液制品中B19病毒的污染情况略低于国外文献报道,可能与试剂盒的灵敏度及样本量有关,有必要对国内的相关制品作进一步的跟踪。Objective To determine the contamination with human parvovirus B19 in source plasma and blood products in China.Methods Primers were designed based on the highly conserved sequence between 2 000 and 2 300 bp in the coding regions of B19 genome,with which individual human plasma samples,plasma pools for production,intravenous immunoglobulin,human fibrinogen,human coagulation factor Ⅶ and human prothrombin complex were dete rmined for B19 DNA by fluorescent quantitative PCR.Results The positive rates of B19 DNA in individual human plasma samples,plasma pools for production,intravenous immunoglobulin,human fibrinogen,human coagulation factor Ⅶ and human prothrombin complex were 0.092%,82.41%,0,45.83%,67.86% and 78.79% respectively.Conclusion The positive rates of B19 virus in source plasma and blood products in China were slightly lower than those reported in the documents abroad,which might be associated with the sensitivity of kit and the quantity of samples.It is necessary to follow the relative message of relevant domestic blood products.

关 键 词：细小病毒B19 人 荧光定量PCR 血液制品 

分 类 号：R457.14[医药卫生—治疗学] R373.9[医药卫生—临床医学]