云南省部分HIV感染者唾液EBV检出率分析  

Detection of Epstein-Barr Virus in the saliva of human Immunodeficiency Virus Infected Patients from Yunnan Province,China

作　　者：史会萍[1,2] 李榕[1,2] 段开文[1,2] 夏志刚[1,2] 高辉 李承文[4,5] 

机构地区：[1]昆明医科大学附属延安医院 [2]昆明市延安医院口腔科,云南昆明650051 [3]昆明市延安医院检验科,云南昆明650051 [4]昆明医科大学第三附属医院 [5]云南省肿瘤医院科研科,云南昆明650118

出　　处：《昆明医学院学报》2012年第6期144-146,160,共4页Journal of Kunming Medical College

基　　金：国家自然科学基金资助项目(30860315;81160135);云南省应用基础研究基金资助项目(2008ZC146M)

摘　　要：目的探讨HIV感染者唾液EB病毒存在情况及其与免疫抑制的关系.方法采用横断面研究,利用巢氏PCR方法对245例HIV感染者和30例健康对照者唾液EBV DNA的存在情况进行检测,并分析检出率与CD4淋巴细胞计数的关系.结果 HIV感染者和健康对照人群唾液EBV检出率分别为82.0%与30.0%,二者有统计学差异(P<0.05);CD4淋巴细胞计数小于200、200～400和大于400个/μL的患者唾液EBV检出率分别为93.0%、75.8%和45.7%,3组间检出率有显著差异(P<0.05);但是否使用高效抗逆转录病毒治疗对唾液EBV的检出率无明显影响.结论 HIV感染者唾液中EB病毒检出率高,与免疫指标CD4淋巴细胞计数水平有关.Objective To determine the presence of Epstein-Barr virus （EBV） -DNA in the saliva from human immunodeficiency virus （HIV） -infected individuals and healthy controls from Yunnan. Methods A cross- sectional study was performed in 245 HIV-infected subjects and 30 healthy controls. EBV DNA in saliva samples was examined by nested polymerase chain reaction （PCR）. Chi-square test was performed to determine the prevalence rates among groups. Results The detection rate of EBV in HIV-infected group and non-HIV controls were 82.0% and 30.0%, respectively. EBV detection rate in HIV-infected subjects were significantly higher than non-HIV controls （P 〈 0.05）. The detection rate of EBV in patients with CD4 counts less than 200 cells/uL, 200-400 cells/ul and above 400 cells/ul were 93.0%, 75.8% and 45.7%, There was a significant difference among them （P 〈 0.05）. No significant difference was observed between the groups HIV-infected subjects who were on HAART and not on HAART （P 〉 0.05）. Conclusion The detection rate of oral EBV in HIV is significantly higher in HIV-infected subjects than non-HIV controls, and it is related to CD4 cell level.

关 键 词：EB病毒 唾液 聚合酶链反应 人类免疫缺陷病毒 

分 类 号：R781.5[医药卫生—口腔医学] R373.9[医药卫生—临床医学]