改良型TAT-VP3融合蛋白对膀胱癌BIU-87细胞Bcl-2和Survivin表达的影响  

The Effect of Modified TAT-VP3 Fusion Protein on the Expression of Bcl-2 and Survivin in Human Bladder Cancer BIU-87 Cells

作　　者：马真[1] 李俊[2] 王剑松[1] 詹辉[1] 王春晖[1] 张之甲[1] 

机构地区：[1]昆明医科大学第二附属医院泌尿外科,云南省泌尿外科研究所,云南昆明650101 [2]昆明医科大学第一附属医院肾内科,云南昆明650032

出　　处：《昆明医学院学报》2012年第7期6-10,共5页Journal of Kunming Medical College

基　　金：国家自然科学基金资助项目(30760251)

摘　　要：目的探讨应用改良型TAT-VP3融合蛋白干预膀胱癌BIU-87细胞后,对肿瘤细胞的抑制效应,及对Bcl-2和Survivin表达的影响,为以后对改良型TAT-VP3融合蛋白的进一步研究提供实验基础.方法运用MTT比色法检测BIU-87细胞的生长抑制率.利用不同浓度的改良型TAT-VP3融合蛋白干预膀胱癌BIU-87细胞,24 h,48 h,72 h,运用实时荧光定量酶链聚合反应(QPCR)检测Bcl-2和Survivin mRNA的表达变化.运用不同浓度的改良型TAT-VP3融合蛋白干预膀胱癌BIU-87细胞,24 h后蛋白免疫印迹法(Western Blo)t检测Bcl-2和Survivin蛋白的表达情况.结果改良型TAT-VP3融合蛋白干预膀胱癌BIU-87细胞后,肿瘤细胞凋亡率与时间和剂量呈依赖关系(P<0.05),Bcl-2和Survivin mRNA表达与时间和药物剂量呈依赖关系.不同浓度的改良型TAT-VP3融合蛋白作用24 h后,Bcl-2和Survivin蛋白表达与药物剂量呈依赖关系.结论改良型TAT-VP3融合蛋白对膀胱癌BIU-87细胞具有一定的抑制作用,并对凋亡相关因子(Bcl-2和Survivin)的表达有影响.Objective To investigate the inhibitory effect of improved TAT-VP3 fusion protein on human bladder cancer BIU-87 cells, and the effect on the expression of bcl-2 and survivin in BIU-87 cells, and provide experiment basis for the further study of improved TAT-VP3 fusion protein. Methods The inhibition rate of cell growth was detected using the method of MTI＇. Different concentrations of improved TAT-VP3 fusion protein were used to interfere with human bladder cancer BIU-87 cells, then the expression of bcl-2 and survivin mRNA was detected by real-time fluorescent quantitative polymerase chain reaction （qPCR） after 24 h, 48 h, 72 h, and the expression of hcl-2 and survivin protein was detected by western blotting after 24 h. Results When improved TAT-VP3 fusion protein interfered with human bladder cancer BIU-87 cells, the inhibition rate of tumor cell growth showed a time- and dose-dependent manner （P 〈 0.05） , and the expression of bcl-2 and survivin mRNA showed a time- and dose-dependent manner, too. When different concentration of improved TAT-VP3 fusion protein interfered with tumor cells, the expression of bcl-2 and survivin protein showed a dose-dependent manner after 24 h. Conclusion Improved TAT-VP3 fusion protein can inhibit the growth of BIU-87 cells in a certain, and influence the expression of bcl-2 and survivin.

关 键 词：细胞凋亡 VP3蛋白 膀胱癌 BCL-2 SURVIVIN 

分 类 号：R737.14[医药卫生—肿瘤]