甲状腺激素对大鼠急性肝衰竭肠粘膜屏障功能的保护作用  

The Protective Effct of Thyroid Hormone on Function of Intestinal Mucosa Barrier in Rats with Acute Hepatic Failure

作　　者：葛玉明 胡敏[2] 顾玲[3] 何建平[3] 郭峰[4] 

机构地区：[1]会泽县妇幼保健院普外科,云南会泽654200 [2]昆明学院医学院,云南昆明650214 [3]昆明医科大学第一附属医院肝胆外科,云南昆明650031 [4]昆明医科大学临床技能中心,云南昆明650031

出　　处：《昆明医学院学报》2012年第7期32-35,共4页Journal of Kunming Medical College

基　　金：国家自然科学基金资助项目(81060361)

摘　　要：目的探讨补充外源性甲状腺激素(thyroid hormone,TH)对急性肝衰竭(acute hepatic failure,AHF)肠粘膜屏障的保护作用.方法 SD大鼠36只,随机分为3组:对照组(SO组)、急性肝衰竭组(AHF组)和治疗组(AHF+TH组).腹腔注射硫代乙酰胺(thioacetamide,TAA)制作AHF模型.15μg/kg的标准腹腔注射三碘甲状腺原氨酸(T3),取下腔静脉血检测TH水平,取小肠组织测二胺氧化酶(DAO)活性,取末段回肠行普通HE染色观察,并使用图像分析系统测量小肠绒毛长度.结果 AHF+TH组较AHF组小肠组织DAO活性升高(P<0.05),小肠绒毛长度升高(P<0.05),肠粘膜损伤程度降低(P<0.05).结论补充外源性TH对AHF时肠粘膜屏障有明显的保护作用.Objective To investigate the role of exogenous thyroid hormone in the protection of intestinal mucosal barrier of acute hepatic failure rats. Methods 36 SD rats were randomly divided into 3 groups： control group （SO group）, acute live failure group （AHF group） and treatment group （AHF＋ TH group）. The AHF Rat model was established by repeated injection of thioacetamide （TAA）. Triiodothyronine （T3） was administered intraperitoneally to correct the low T3 syndrome of AHF rats. The blood in inferior vena cava was collected to detect the level of thyroid hormone, the activity of diamine oxidase （DAO） in small intestine was detected, and the pathological structure of the end of ileum was observed by HE staining, the length of intestinal villi was determined by image analysis system. Results Compared with AHF group, the activity of DAO in in small intestine was significantly increased （P 〈 0.05） , the length of intestinal villi was shortened （P 〈 0.05） , and the degree of intestinal mucosal injury was decreased. Conclusion Thyroid hormone might play roles in the protection of intestinal mucosal barrier.

关 键 词：急性肝衰 肠粘膜屏障 甲状腺激素 

分 类 号：R735.7[医药卫生—肿瘤]