甲状腺激素对大鼠急性肝衰竭肠粘膜屏障功能影响的实验研究  

The Protective Effct of Thyroid Hormone on Function of Intestinal Mucosa Barrier in Rats with Acute Hepatic Failure

作　　者：郭峰 胡敏 何建平[3] 顾玲[3] 储平坤 

机构地区：[1]昆明医科大学临床技能中心,云南昆明650031 [2]昆明学院医学院,云南昆明650214 [3]昆明医科大学第一附属医院肝胆外科,云南昆明650031 [4]昆明市呈贡区人民医院普外科,云南呈贡650500

出　　处：《昆明医学院学报》2012年第7期41-44,共4页Journal of Kunming Medical College

基　　金：国家自然科学基金资助项目(81060361);云南省应用基础研究基金资助项目(2010zc169)

摘　　要：目的探讨急性肝衰竭(acute hepatic failure,AHF)时肠粘膜屏障功能损害及甲状腺激素(thyroidhormone,TH)代谢异常,补充外源性甲状腺激素对损害的肠粘膜屏障的影响.方法 SD大鼠34只,随机分为3组:正常对照组(SO组)、急性肝衰竭组(AHF组)和治疗组(AHF+TH组).腹腔内注射硫代乙酰胺(thioac-etamide,TAA)模拟建立AHF模型.按15μg/kg的标准腹腔注射TH,取下腔静脉血检测TH水平及肝功能指标,取门静脉血测内毒素水平,取肝脏行普通HE染色观察,并使用图像分析系统测量小肠绒毛长度.结果 AHF+TH组肝功能较AHF组有改善(P<0.05),内毒素水平有所降低,小肠绒毛长度有所升高(P<0.05).结论补充外源性TH在AHF时有一定的保护肝脏功能和减轻损伤、保护肠粘膜屏障的作用.Objective To investigate the injury of intestinal mucosa barrier, the metabolic abnormalities of thyroid hormone, and the protective effect of exogenous thyroid hormone on intestinal mucosa barrier in rats with acute hepatic failure. Methods 36 SD rats were randomly divided into 3 groups： control group （SO group） , acute live failure group （AHF group） and treatment group （AHF＋TH group）. The AHF Rat model was established by repeated injection of thioacetamide （TAA）. TH was administered intraperitoneally to correct the low TH syndrome of AHF rats. The blood in inferior vena cava was collected to detect the level of thyroid hormone and liver function, the blood in portal vein was collected to detect the level of endotoxin, and the liver was sampled to observe the pathological structure by HE staining, the length of intestinal villi was determined by image analysis system. Results Compared with AHF group, the liver function of rats in AHF＋ TH group was significantly improved （P 〈 0.05） , the blood levels of endotoxin were decreased and the length of intestinal villi was extended （P 〈 0.05）. Conclusion The supplement of exogenous thyroid hormone can protect intestinal mucosa barrier and the function of liver, and alleviate the injure of intestinal mucosa barrier in rats with acute hepatic failure.

关 键 词：急性肝衰 肠粘膜屏障 甲状腺激素 

分 类 号：R735.7[医药卫生—肿瘤]