间充质干细胞对大鼠角膜移植排斥反应作用的观察  

Investigation of the role of mesenchymal stem cells on keratoplasty rejection

作　　者：张晓敏[1] 焦春娜[1] 贾喆[1] 任新军[1] 李筱荣[1] 赵少贞[1] 

机构地区：[1]天津医科大学眼科中心,300384

出　　处：《中华眼科杂志》2012年第8期733-738,共6页Chinese Journal of Ophthalmology

基　　金：国家自然科学基金（30901656）;天津市自然科学基金（07JCYBJC16500）;天津市自然科学基金重点项目（11JCZDJC19600）

摘　　要：目的研究尾静脉输注间充质干细胞（MSC）对大鼠角膜移植免疫排斥反应的作用。方法实验研究。原代培养Wistar大鼠骨髓MSC，达90％融合时传代培养。制作大鼠角膜移植动物模型，以Lewis大鼠为受体，Wistar大鼠为供体，随机分为A、B、C三组。A组给予1ml不含MSC的PBS，B组术前连续3d输注MSCs，C组术后当天开始连续3d输注MSC。裂隙灯显微镜下对角膜植片进行临床观察，比较2组角膜植片的平均存活时间。A组与C组部分大鼠于术后10d行角膜免疫组化检测角膜植片CD4、CD8、CD25和CD45的表达水平。应用Kaplan—Meier检验比较3组植片平均存活时间。免疫组化数据采用单因素方差分析及两样本均数比较的t检验。结果C组角膜植片的存活时间为（9．9±0．69）d，与A、B组[（11．83±0．54），（16．89±1．91）d]相比均有明显延长（F=5．732；P=0．001，0．019）。B组角膜植片存活时间较A组相比延长，但无统计学意义（P=0．333）。C组CD4、CD8、CD25的表达水平显著低于A组（t=2．477，2．359，2．445；P=0．024，0．042，0．030）。结论术后输注MSC能显著延长大鼠同种异体角膜移植片存活时间，对大鼠角膜移植排斥反应有抑制作用。Objective To investigate the effect of mesenchymal stem cells （MSC） on keratoplasty rejection in a rat mode. Methods MSC from bone marrow of Wistar rats was cultured. Corneas of Wistar rats （donors） were transplanted to Lewis rats （recipients）. Transplanted rats were randomly divided into A, B, C three groups. Rats in group B were injected with MSC suspended in PBS via the tail vein continually for three days before the surgery, while rats in group C accepted similar MSC transplantation after the surgery. The rats in group A were given the same volume of PBS. Grafts were scored for corneal transparency, edema and extent of neovascularization by slit lamp observation. Expressions of CD4 , CDs , CD25 and CD45 in corneas 10 days after transplantation were examined by immunohistochemistry. Results The survival time of the corneal grafts in group C [ （9. 9 ± 0. 69） d ] was significantly prolonged compared with that of the group A and group B [（11.83 ±0.54）, （16.89±1.91）d] （F=5.732,P=0.001, 0. 019 ）. Expression level of CD4 ,CD8 ,CD25 of the corneal grafts in group C was lower than that of group A and group B （t = 2. 477, 2. 359, 2. 445,P = O. 024, 0. 042, 0. 030）. Conclusion Post-operative injection of MSCs could inhibit keratoplasty rejection and prolong the corneal allografts suivival in a rat model.

关 键 词：角膜移植 移植物排斥 间质干细胞 

分 类 号：R779.65[医药卫生—眼科]