冷冻睾丸精子行卵泡浆内单精子注射术的结局评估  

Analysis of outcomes of cryopreserved testicular sperm from OA for ICSI

作　　者：汪李虎[1] 杨少芬[1] 杨旭辉[1] 陈创奇[1] 黄郁强[1] 易艳红[1] 

机构地区：[1]广东省妇幼保健院,广州510010

出　　处：《生殖医学杂志》2012年第4期338-340,共3页Journal of Reproductive Medicine

基　　金：广东省省科技厅基金(2006B36003016);广东省卫生厅基金(A2006091)

摘　　要：目的梗阻性无精子症患者采用诊断性睾丸精子抽吸术(TESA),对获得的睾丸精子进行冷冻,评估新鲜精子和冷冻精子分别行卵泡浆内单精子注射术(ICSI)的受精率和妊娠率。方法回顾分析本院2007～2010年就诊的237对梗阻性无精子症(OA)夫妇用新鲜睾丸精子行ICSI和2010～2011年就诊的31例OA患者的冷冻睾丸精子行ICSI后胚胎移植的资料。结果新鲜精子组和冷冻精子组的平均成熟卵泡数分别为(14.13±7.41)和(14.31±8.14),平均正常受精率分别为82.99%和84.17%,种植率分别为24.08%和25.64%,以上指标两组相比较均无显著性差异(P>0.05)。两组的临床妊娠率也没有显著差异(新鲜精子组43.88%和冷冻精子组48.39%,P>0.05)。结论冷冻睾丸精子不影响ICSI的受精率和临床妊娠率,采用TESA获取的冷冻睾丸精子行ICSI是一种能减少患者痛苦又安全、有效的方法。Objective： To evaluate the fertilization and pregnancy rates for in vitro intracytoplasmic sperm injection （ICSI） using either cryopreserved testicular sperm or fresh sperm obtained from the patients with obstructive azoospermia（OA） by surgery. Method： A total of 268 couples including 237 couples using fresh testicular sperm and 31 couples using frozen-thawed testicular sperm were performed with IVF/ICSI cycles in our hospital from 2007 to 2010. The data were retrospective analyzed. Results. The number of mature oocytes retrieved （14.13：k7.41 vs. 14.31~8.14）, fertilization rate （82.99% vs. 84.17~）, implantation rate （24.08% vs. 25.64~） and clinical pregnancy rates （43.88% vs. 48.39 %） were not significantly different between the fresh and frozen-thawed sperm groups. Conclusions. These results showed that application of frozen-thawed testicular sperm does not appear to reduce fertilization or clinical pregnancy rates. ICSI using frozen-thawed testicular sperm from obstructive azoospermia patients is an effective and safe method.

关 键 词：梗阻性无精子症 睾丸精子 冷冻 妊娠结局 

分 类 号：R[医药卫生]