IGF-Ⅰ对奶牛乳腺上皮细胞合成乳脂、乳蛋白的影响  

IGF-Ⅰ regulating synthesis of milk fat and milk protein in bovine mammary epithelial cells

作　　者：何伯萍[1] 邢艳苹[1] 雷连成[1] 韩东[1] 李莹莹[1] 吴云娣[1] 杨峰[1] 

机构地区：[1]吉林大学畜牧兽医学院,吉林长春130062

出　　处：《中国兽医学报》2012年第8期1231-1234,共4页Chinese Journal of Veterinary Science

基　　金：国家重点基础研究发展计划("973"计划)资助项目(2011CB100805)

摘　　要：分离培养奶牛乳腺上皮细胞,添加不同质量浓度的IGF-Ⅰ(insulin-like growth factor-Ⅰ)(0,10,100,200μg/L)刺激24h后,提取细胞总RNA,用荧光定量RT-PCR方法检测β-酪蛋白(CSN2)和乙酰辅酶A羧化酶α(ACA-CA)基因的转录水平变化。结果显示,IGF-Ⅰ能够显著促进CSN2和ACACA基因的转录,并且具有浓度依赖性。结果表明,IGF-Ⅰ可作为信号分子调节乳腺的泌乳功能。Isolated and cultured bovine mammary epithelial cells,we treated the dells with different concentrations of IGF-I （insulin-like growth factor-I ） （0,10,100,200 μg/L）. After 24 h, total RNA was extracted from the cells. The gene transcription levels of beta-casein （CSN2） and acetyl coenzyme A earboxylase alpha （ACACA） were detec- ted with real-time PCR method. The results showed that IGF-I could significantly promoted CSN2 and the ACACA gene transcription with a concentration dependent manner. The results indicated that 1GF- I may be as a signaling molecule to regulate mammary gland lactation.

关 键 词：奶牛乳腺上皮细胞 IGF-Ⅰ Β-酪蛋白 乙酰辅酶A羧化酶α 

分 类 号：S823.9[农业科学—畜牧学] S852.4[农业科学—畜牧兽医]