5-FU诱导人肝细胞癌MDR的比较蛋白质组学研究  

Comparative proteomics analysis of human hepatocellular carcinoma MDR induced by 5-FU

作　　者：韦艳 张海英[2] 梁钢[2] 

机构地区：[1]武警广西总队医院,广西南宁530003 [2]广西医科大学药学院,广西南宁530021

出　　处：《基础医学与临床》2012年第9期1015-1020,共6页Basic and Clinical Medicine

基　　金：国家自然科学基金(30760310);广西地方性高发疾病防治研究重点实验室基金(桂科能0842009-k6);广西科学基金(桂科自0728113);广西中医药研究院中药质量标准研究重点实验室开放课题基金(桂中重开0801)

摘　　要：目的寻找与人肝细胞癌多药耐药相关的蛋白质分子,为进一步研究人肝细胞癌多药耐药机制提供依据。方法体外培养人肝癌细胞Bel-7402及其耐5-氟尿嘧啶(5-FU)细胞Bel-FU,MTT法检测Bel-FU的多药耐药性,双向凝胶电泳技术分析细胞Bel-7402与Bel-FU之间的差异表达蛋白,经MALDI-TOF/TOF质谱鉴定。Western blot验证2-DE及质谱的检测结果。结果与Bel-7402比较,Bel-FU中有24个蛋白表达上调,其中包括FAK、TM3、ORP150、CRT、hnRNP K、80K-H protein、EF-1-D、phosphoprotein等,12个蛋白表达下调。Western blot法检测到蛋白FAK、CRT在Bel-FU中高表达,与2-DE结果一致。结论通过建立人肝癌细胞Bel-7402及其耐5-氟尿嘧啶细胞Bel-FU的2-DE图谱筛选了多药耐药相关的差异蛋白,为人肝细胞癌MDR的分子机制研究奠定了基础。Objective To search the protein from human hepatocellular carcinoma MDR and provide evidence for the mechanism of hepatocellular carcinoma MDR. Methods Human hepatocellular carcinoma cell line Bel-7402 and 5-FU resistant cell line Bel-FU were cultured. The MDR of BeI-FU was detected by MqT assay, to screen the differential expression protein between Bel-7402 and Bel-FU by 2-DE and MALDI-TOF/TOF. Results Compared with those in Bel-7402, 24 proteins were up-regulated in Bel-FU including FAK, TM3, ORP150, CRT, hnRNP K, 80K-H protein, EF-1-D, phosphoprotein and so on,and 12 proteins were down-regulated. Western blot confirmed that FAK and CRT were up-regulated in Bel-FU, the results were in accordance with the results of 2-DE. Conclu- sions The differential expression protein between Bel-7402 and BeI-FU may support the study of molecule mecha- nism of MDR.

关 键 词：肝细胞癌 多药耐药 比较蛋白质组学 双向凝胶电泳 

分 类 号：R735.7[医药卫生—肿瘤]