大鼠脑出血后V_(1α)R和AQP4表达升高与脑水肿的形成相关  

Increased expression of V_(1α) receptor and AQP4 are related to rat brain edema after intracerebral hemorrhage

作　　者：张波[1] 孙善全[1] 甘胜伟[1] 钟小燕[1] 伍修宇[1] 徐进[1] 黄娟[1] 

机构地区：[1]重庆医科大学神经科学研究中心,重庆400016

出　　处：《基础医学与临床》2012年第9期1021-1025,共5页Basic and Clinical Medicine

基　　金：国家自然科学基金(30470608)

摘　　要：目的研究精氨酸加压素1α受体(V1αR)和水通道蛋白4(AQP4)在SD大鼠脑出血(ICH)脑组织中的表达变化及两者在脑水肿形成中的作用。方法将自体血注入大鼠尾状核建立脑出血模型,分别于术后6、12、24和48 h运用干湿重法、免疫荧光和Western blot检测脑含水量(BWC)、V1αR和AQP4蛋白的表达。结果脑出血后6 h,V1αR和AQP4的表达开始增加,至48 h达到高峰,分别为21.88±0.44和23.16±0.67,显著高于假手术组(14.32±0.55和13.90±0.40),与脑含水量呈正相关(P<0.05)。结论 V1αR介导的AQP4表达升高是脑出血后脑水肿形成的主要原因。Objective To investigate the change of arginine-vasopressin Via receptor （V1α R）and aquaporin 4 （AQP4） expression, to explore the mechanism of brain edema caused by intracerebral hemorrhage（ICH）. Methods Autologous blood were injected into the caudate nucleus to develope ICH model. The changes of brain water content （BWC）were measured by wet and dry weight methods. The expression of V1αR and AQP4 were detected by immu- nofluorescence and Western blot after 6, 12, 24 and 48 h. Results The expression of V1αR and AQP4 increased at 6h after ICH ,and significantly increased the peak at 48 h （21.88± 0. 44 and 23.16 ± 0. 67 ）than the sham group （ 14. 52± 0. 55 and 15.90 ± 0. 40）. V1α R and AQP4 expressions positively correlated with BWC （ P 〈 0. 05 ）. Conclusions The increased expression of AQP4 mediated by V1αR plays an important role in the brain edema after ICH.

关 键 词：精氨酸加压素 V1α受体 水通道蛋白4 脑水肿 

分 类 号：R743.3[医药卫生—神经病学与精神病学]