RB1和D13S319探针检测慢性淋巴细胞白血病13q14缺失  

DNA Probe into RB1,D13S319 for Determination of 13q14 Deletion in Chronic Lymphocytic Leukemia

作　　者：郭明权[1] 王志林[2] 汪慧芸[3] 

机构地区：[1]江阴市人民医院,江苏江阴214400 [2]常州市第一人民医院,江苏常州213003 [3]江阴市中医院,江苏江阴214400

出　　处：《中国血液流变学杂志》2012年第2期189-190,258,F0002,共4页Chinese Journal of Hemorheology

摘　　要：目的研究慢性淋巴细胞白血病（CLL）13q14缺失的情况。方法运用位于13q14的序列特异性DNA探针RB1、D13S319和间期荧光原位杂交（I-FISH）技术对39例初发的CLL患者进行染色体13q14的检测。结果B-CLL中16例（41.0％）有13q14缺失，阳性细胞率为23.O％～89.0％，其中RB1单独缺失0例，D13s319单独缺失13例（33.3％），RB1、D13S319同时缺失3例（7.7％）。结论CLL患者13q14缺失以D13S319为主，荧光原位杂交技术是研究慢性淋巴细胞白血病13q14缺失快速而可靠的方法。Objective To investigate the incidence of 13q14 deletion[del（13ql4）] in chronic lymphocytic leukemia（CLL）.Metbods Sequence-specific DNA probes into RB1,D13S319 for 13q14 and fluorescence in situ hybridization（I-FISH） were utilized to detect del（13q14） in 39 newly diagnosed patients with B-CLL. Results 13q14 deletion occurred in 16 cases of all patients with B-CLL（41.0%）,in which the percentage of positive cells was 23.0%～ 89.0%.Among these patients with 13q14 deletion,there were 13 patients（33.3%） with D 13 $319 deletion and 3 patients（7.7%） with both RB 1 deletion and D 13 $319 deletion,but none of them had RB 1 deletion.Conclusion D13S319 deletion occupies a leading position during CLL patients with 13q14 deletion. In addition,fluorescence in situ hybridization can be used as a rapid and reliable method for the determination of 13q14 in chronic lymphocytic leukemia.

关 键 词：慢性淋巴细胞白血病 13q14 荧光原位杂交 

分 类 号：R733.72[医药卫生—肿瘤] R446.7[医药卫生—临床医学]