TSP-1在肾透明细胞癌与外周血中的表达及临床意义  

The Expression and Clinical Significance of TSP-1 in Human Clear Cell Renal Cell Carcinoma and Peripheral Blood

作　　者：邱振伟[1] 温端改[1] 侯建全[1] 浦金贤[1] 张光波[1] 魏雪栋[1] 

机构地区：[1]苏州大学附属第一医院泌尿外科,江苏苏州215006

出　　处：《中国血液流变学杂志》2012年第2期205-208,249,共5页Chinese Journal of Hemorheology

摘　　要：目的检测并分析凝血酶敏感蛋白-1（thrombospondin-1，TSP-1）在。肾透明细胞癌患者肾癌、癌旁组织、正常肾组织及外周血中的表达及临床意义。方法收集47例肾透明细胞癌患者的标本包括：47例。肾癌组织、41例癌旁组织、38例正常肾组织、35例患者外周血标本，制备组织抽提液及外周血清。运用ELISA法检测三种组织及血清中TSP-1的表达，并结合临床资料分析其临床意义。结果TSP-1在肾癌组织、癌旁组织、正常肾组织三者间的表达水平分别为36．44±4．80ng／mL、42．55±4．12ng／mL、46．55±1．55ng／mL，依次递增，三种组织间的表达水平，两两比较均有统计学差异（P均〈0．01）。TSP-1在肾癌组织中的表达，随着肾癌TNM分期与Fuhrman分级的提高而均降低，且都呈负相关（r=-0.84，P〈0．01；r=-0.70，P〈0.01）。结论TSP-1在肾透明细胞癌组织中低表达，可能在抑制肿瘤血管形成过程中起重要作用；TSP-1在肾透明细胞癌组织中的表达与肾癌的TNM分期和Fuhrman分级关系密切，可作为评判。肾透明细胞癌发展及恶性程度的指标。Objective To detect and analyze the expression and clinical significance of thrombospondin-1（TSP-1）,in healthy persons＇ peripheral blood,clear cell renal cell carcinoma（ccRCC） tissue,cancer adjacent tissue,normal renal tissue and pre-operation peripheral blood of human clear cell renal cell carcinoma patients. Methods We collected 47 cases of ccRCC tissue,41 cancer adjacent tissue,38 normal renal tissue and 35 preoperation peripheral blood samples of ccRCC patients.We used Enzyme-linked immunosorbent assays（ELISA） to determine the expression of TSP-1 in the three tissues extracts and peripheral serum samples,and analyzed their clinical significances.Results The expression of TSP-I in ccRCC tissue,cancer adjacent tissue and normal renal tissue were 36.44 ± 4.80ng/mL,42.55 ± 4.12ng/mL,46.55 ± 1.55ng/mL respectively, which was successively increasing.Both of them was all statistical significance（Pall 〈 0.01）.The expression of TSP-1 was negatively correlated with the TNM stage and Fuhrman grade of RCC（r= - 0.84,P 〈 0.01 ;r= - 0.70,P 〈 0.01）.The expression of TSP-1 was no statistical significance with the age,the sexual of patient＇s and the size of tumor（Pall 〉 0.05）. There were no statistical significance in TSP-1 expression between patient＇s peripheral blood sample and healthy person＇ s blood sample （170.3 ± 20.04ng/mL vs 170.0 ±18.06ng/mL,P=O.62）.Conclusions The low expression of TSP-1 may be important factor in inhibiting tumor angiogenesis.The expression level of TSP-1 was closely related with TNM stage and Fuhrman grade in RCC which can be used as two indicators to evaluate the development andgrade malignancy of tumor.

关 键 词：肾透明细胞癌 肾细胞癌 凝血酶敏感蛋白-1 酶联免疫吸附法 

分 类 号：R737.11[医药卫生—肿瘤]