富含BCAA的PN对肝硬化大鼠肝部分切除术后肝脏NK的影响  

Effect of Parenteral Nutrition with Branch-Chain Amino Acids Enrichment on the Natural Killer Cells in the Liver after Partial Hepatectomy in Rats with Liver Cirrhosis

作　　者：张雷[1] 罗时敏[2] 张晓坤[1] 黄国威[1] 廖一鸣[1] 

机构地区：[1]广州医学院附属荔湾医院,广东广州510170 [2]广州市第一人民医院,广东广州510180

出　　处：《现代生物医学进展》2012年第23期4442-4443,4529,共3页Progress in Modern Biomedicine

基　　金：广州市荔湾区科技计划项目(20071209112)

摘　　要：目的:探讨富含支链氨基酸的肠外营养对肝硬化大鼠肝部分切除术后肝脏自然杀伤细胞(NK细胞)的影响。方法:20只肝硬化大鼠随机分为肝部分切除术后行8.5%Novamin的肠外营养5 d组10只,肝部分切除术后行10%Hepa的肠外营养5 d组10只。应用流式细胞仪测定大鼠肝脏NK细胞的百分率;应用4小时51Cr释放法测定肝脏NK细胞的杀伤活性。结果:与8.5%Novamin的肠外营养5 d组比较,10%Hepa的肠外营养5 d组肝脏NK细胞占全部淋巴细胞的百分比和肝脏NK细胞杀伤活性明显升高(P<0.05)。结论:富含支链氨基酸的肠外营养可以增加肝脏NK细胞的百分率和肝脏NK细胞的杀伤活性。Objective： To explore the effect ofparenteral nutrition （PN） with branch-chain amino acids enrichment on the natural killer cells in the liver after partial hepatectomy in rats with liver cirrhosis. Methods： Twenty rats with liver cirrhosis were randomly divided into post-operation 8.5% Novamin PN 5 day group （n=10） and post-operation 10% Hepa PN 5 day group （n=10）. Activity of NK cells was measured by 4h 51Cr-release assay. The percentages of the NK cells in the livers was examined by flow cytometry. Results： Compared with the 8.5% Novamine PN 5 day group, the percentages and the activity of NK cells in the livers were significantly increased in the 10%Hepa PN 5 day group （P〈0.05）. Conclusion： Parenteral nutrition with branch-chain amino acids enrichment after partial hepatectomy in rats with liver cirrhosis can ameliorate the percentages and the activity of NK cells in the livers.

关 键 词：肝硬化 肝切除术 支链氨基酸 自然杀伤细胞 

分 类 号：Q953[生物学—动物学] R575.2[医药卫生—消化系统]