VEGF-C、VEGF-D及其受体Flt-4在胰腺癌中的表达及意义  

EXPRESSION AND SIGNIFICANCE OF VEGF-C,VEGF-D AND IT'S RECEPTOR Flt-4 IN HUMAN PANCREATIC CARCINOMA

作　　者：李智峰[1] 李高强[1] 田云霄[2] 袁艳龙[2] 司华鹏[1] 梁冰锋[3] 

机构地区：[1]河北省邯郸市第一医院普外一科,河北邯郸056002 [2]河北省邯郸市中心医院病理科,河北邯郸056001 [3]河北女子职业技术学院护理系,河北石家庄050091

出　　处：《河北医科大学学报》2012年第8期890-893,F0002,共5页Journal of Hebei Medical University

基　　金：河北省科技厅科学技术支撑项目(09276153)

摘　　要：目的探讨血管内皮生长因子(vascular endothelial growth factor,VEGF)-C、VEGF-D及fms-liketyrosinekinase-4(Flt-4)蛋白在胰腺癌组织中的表达及与胰腺癌临床病理特征之间的关系。方法选择根治性手术切除的胰腺癌组织63例及正常胰腺组织13例。采用免疫组织化学法(Envision法)检测胰腺癌组织及正常胰腺组织中VEGF-C、VEGF-D的表达,检测癌周组织及正常胰腺组织中Flt-4的表达,并结合患者的临床病理特征进行统计学分析。结果 63例胰腺癌组织中VEGF-C阳性表达38例(60.3%)、VEGF-D阳性表达40例(63.5%)、癌周Flt-4阳性表达41例(76.2%),均高于正常胰腺组织(P<0.05)。VEGF-C、VEGI-D和Flt-4的高表达均与淋巴结转移及患者生存率密切相关(P<0.05)。胰腺癌中VEGF-C、VEGF-D与癌周Flt-4蛋白的表达呈正相关(P<0.05)。结论 VEGF-C、VEGF-D在胰腺癌中表达增高,Flt-4在癌周组织中表达增高,表明它们与胰腺癌的生物学侵袭性行为有关。在胰腺癌中VEGF-C、VEGF-D均与Flt-4的表达呈显著正相关,并与淋巴结转移及患者生存期密切相关,表明VEGF-C、VEGF-D和Flt-4可以促使胰腺癌发生淋巴结转移,且在转移过程中有相互促进作用。Objective To evaluate the expression of vascular endothelial growth factor （VEGF） - C, VEGF - D and flns - like tyrosine kinase - 4 （ Fit - 4 ） in pancreatic cancer, and analyze the relationships between the expression and the clinicopathological characteristics of pancreatic carcinoma. Methods Immunohistochemistry was used to detect the expression of VEGF - C, VEGF - D and Fit -4. Statistical analysis was used to determine the relationship between the expression and clinicopathologieal parameters of pancreatic Carcinoma. Results The positive rates of VEGF - C and VEGF- D in pancreatic carcinoma were 60.3% （38/63）and 63.5% （40/63）, which were significantly higher than the positive rates 23.1% （3/13）and 23.1% （3/13）in normal pancreatic tissue respectively （P 〈 0.05 ）. The positive rates of Fit - 4 in paraneoplastic and normal pancreatic tissue were 76.2% （41/63） and 30.8% （4/13） respectively （ P 〈 0.05 ）. The expressions of VEGF - C and VEGF - D in pancreatic carcinoma were correlated positively with the expressions of Flt- 4 in paraneoplastic tissue （ P 〈 O. 05 ）. The overexpression of VEGF - C, VEGF - D and Fh - 4 in pancreatic cancer were correlated significantly with lymph node metastasis and survival time of patients （P 〈 O. 05 ）. Cortelusion The expression of VEGF - C and VEGF -~ D in pancreatic cancer increases, so does the expression of Fit - 4 in cancer tissues, which indicate that they are related to the biology invasive behavior of pancreatic cancer. In pancreatic cancer, both the expression of VEGF - C and VEGF - D are positively correlated with the expression of Fit- d significantly, furthermore, they are closely linked with lymph node metastasis and patient survival, indicating that VEGF - C, VEGF - D and Fit -4 can provide pancreatic cancer with lymph node metastasis, and have mutual promotion in the process of metastasis.

关 键 词：胰腺肿瘤 血管内皮生长因子类 免疫组织化学 

分 类 号：R735.9[医药卫生—肿瘤]