等电聚焦电泳测定草菇低温诱导蛋白等电点的研究  

STUDY ON THE ISOELECTRIC POINT OF LOW TEMPERATURE INDUCED PROTEIN OF STRAW MUSHROOM MEASURED BY ISOELECTRIC FOCUSING ELECTROPHORESIS

作　　者：赵安庆[1] 尹国盛[2] 张蒙[3] 

机构地区：[1]河南农业大学基础科学学院,河南郑州450002 [2]河南大学物理系,河南开封475000 [3]河南省卫生防疫站环境卫生科,河南郑州450003

出　　处：《郑州粮食学院学报》2000年第2期78-80,共3页Journal of Zhengzhou Institute of Technology

摘　　要：利用等电聚焦电泳 (IEF)技术测定了草菇低温诱导蛋白的等电点 ,分别用宽范围pH3～1 0和窄范围pH5～ 8两次电泳 ,精确测定出该蛋白的等电点为 6.79,pH梯度曲线的相关系数r达 0 .937.The isoelectric point of low temperature induced protein of straw mushroom was measured by isoelectric focusing electrophoresis with wide range pH3～10 and narrow range pH5～8 respectively.The isoelectric piont of the protein is 6.79,and the coefficients r of pH gradient curve is 0.937.

关 键 词：等电聚焦电泳 草菇低温诱导蛋白 等电点 食用菌 

分 类 号：S646.13[农业科学—蔬菜学] O657.15[农业科学—园艺学]