肺腺癌组织与癌旁组织长链非编码RNA表达谱的筛选  

Changes of long non-coding RNA expression profiling between pulmonary adenocarcinoma tissue and paracancerous tissue

作　　者：周雪峰[1] 但攀[1] 朱明林[1] 张力[1] 杨泽天[1] 马麒[1] 赵金平[1] 

机构地区：[1]武汉大学中南医院胸心外科,430071

出　　处：《中华实验外科杂志》2012年第8期1489-1490,共2页Chinese Journal of Experimental Surgery

基　　金：国家自然科学基金资助项目（81101775）;湖北省卫生厅年度科研资助项目（NX2011-6）;中央部属高校专项科研基金资助项目（111118）

摘　　要：目的应用高通量长链非编码RNA（1ncRNA）芯片检测肺腺癌组织和癌旁组织中差异表达的IncRNA，并对其进行初步分析。方法分别提取6例肺腺癌患者的肺腺癌组织和癌旁组织中的RNA，体外逆转录制备并标记为双链cDNA（ds—cDNA），与含有22000条lncRNA芯片进行杂交，计算机扫描并分析芯片荧光信号图像，筛选差异表达的lncRNA。结果在肺腺癌组织中差异表达的lncRNA达1025条，其中在肿瘤组织中高表达的IncRNA464条，低表达的IncRNA561条（变化〉2倍且P〈0．05）。结论肺腺癌的发生过程中IncRNA的表达谱发生明显变化，IncRNA与肺腺癌的发生密切相关。Objective To test the differentially expressed long non-coding RNA （lncRNA） in the human pulmonary adenocarcinoma tissue and the paracancerous normal lung tissue by using lncRNA ex- pression microarray. Methods Total RNA from the pulmonary adenocarcinoma tissue and paracancerous normal lung tissue of 6 patients with pulmonary adenoearcinoma was prepared respectively, and ds-cDNA was synthesized and labeled. Hybridization was performed with the profile chip containing more than 22 000 human lncRNAs. Data were analyzed by using the Agilent GeneSpring GX software. Results Of 22000 lncRNAs, about 1025 lncRNAs were differentially expressed in pulmonary adenocarcinoma tissue, in which 464 lncRNAs were up-regulated and 561 lncRNAs were down-regulated as compared with the para- cancerous normal lung tissue. Conclusion Obvious changes of lncRNA expression profiling were observed in the pathogenesis of pulmonary adenocarcinoma, lncRNA may be related to the progress of pulmonary adenocarcinoma.

关 键 词：肺腺癌 长链非编码RNA 芯片 

分 类 号：R734.2[医药卫生—肿瘤]