转化生长因子-β1诱导的半胱氨酰天冬氨酸特异性蛋白酶依赖的人胃癌细胞凋亡途径  

The effects of transforming growth factor-β1 induced apoptosis of human gastric carcinoma cells related to Caspases

作　　者：解刚强[1] 刘德纯[1] 陈登庭[1] 邓淼[1] 汪建光[1] 刘起鹏[1] 

机构地区：[1]河南科技大学第一附属医院普外科,洛阳471003

出　　处：《中华实验外科杂志》2012年第8期1553-1555,共3页Chinese Journal of Experimental Surgery

摘　　要：目的探讨转化生长因子-β1（TGF—β1）诱导胃癌细胞的凋亡作用及机制。方法利用TGF-β1诱导胃癌细胞SGC7901凋亡，小干扰RNA（siRNA）封闭半胱氨酰天冬氨酸特异性蛋白酶（Caspase）-8的表达，Westernblot检测诱导及封闭前后各Caspases表达随时间的变化，流式细胞术分析细胞的凋亡率。结果TGF—β1作用后，各组细胞的凋亡率明显增加，0、24、48h凋亡率分别为1．13％、14．88％、37．32％；Caspase-812h后激活，Caspase-924h后激活；白RNA干扰（RNAi）后Caspase-8表达明显下降，24h后Caspase-8表达量为对照组的11．2％，48～72h未测到表达，96h后Caspase-8表达重新出现，为对照组的9．2％。结论TGF—B1通过Caspase途径诱导胃癌细胞发生凋亡，由Caspase-8开始启动；抑制Caspase-8表达可以在一定程度上阻断TGF—β1／Caspases信号的转导，进而抑制细胞凋亡。Objective To investigate the effects of transforming growth factor-β1 （ TGF-β1 ） in- duced apoptosis. Methods Apoptosis proportion of human gastric SGC7901 carcinoma cells induced by TGF-β1 was tested by flow cytometry, blocking Caspase-8 by RNA interference （RNAi） , the expression of Caspase-8 was analyzed by Western blotting. Results The apoptosis rate of SGC7901 cells obviously increased with the treatment of TGF-β1, an active fragment of caspase-8 started to occur 12 h after TGF-β1 treatment, whereas the caspase-9 active fragment occurred 24 h after TGF-β1 treatment. After RNAi a- gainst Caspase-8, the expression of Caspase-8 was decreased, 11.2% of control group at 24 h, and then can not be detected at 48 h, 72 h, re-emergence at 96 h, 9.2% of control group. Conclusion TGF-β1 induced the apoptosis of SGC7901 cells via Caspases pathway, caspase-8 may be the first easpase activa- ted;Suppression of caspase-8 by RNAi can effectively block the TGF-β1/Caspases signal pathway, and subsequently inhibits the apoptosis of SGC7901 cells.

关 键 词：胃肿瘤 转化生长因子-Β1 半胱氨酰天冬氨酸特异性蛋白酶 细胞凋亡 

分 类 号：R735.2[医药卫生—肿瘤]