酸感受离子通道激活／抑制对大鼠痫性发作的影响  

Acid-sensor ion channels activation/inhibition on rats with lithium pilocarpine-induced epilepsy

作　　者：梁静静[1] 冯莹[1] 陈谦学[2] 潘松青[1] 卢祖能[1] 肖哲曼[3] 

机构地区：[1]武汉大学人民医院神经精神病研究所神经内科研究室,430060 [2]武汉大学人民医院神经精神病研究所神经外科研究室,430060 [3]武汉大学人民医院神经精神病研究所神经内科Ⅱ区,430060

出　　处：《中华实验外科杂志》2012年第8期1566-1568,共3页Chinese Journal of Experimental Surgery

基　　金：国家自然科学基金资助项目（30900459）;武汉大学青年教师资助项目（3082012）;国家教育部新教师基金资助项目（090392）;湖北省教育厅科学技术研究计划指导性项目（B类）（B20090102）

摘　　要：目的观察酸感受离子通道（ASICs）激活／抑制对氯化锂一匹罗卡品大鼠的癫痫皮层脑电图癫痫放电的影响。方法制作匹罗卡品大鼠癫痫模型，分别腹腔注射磷酸盐缓冲液（PBS）、阿米洛利、左乙拉西坦或酸性液体，记录脑电图癫痫样发电次数、波幅、放电间期变化。结果在注射后60～90min，与正常对照组比较，ASICs抑制组癫痫样放电次数（0．69±0．08）、波幅（0．70±0．16）均明显下降（P〈0．05），放电间期（1．46±0．18）延长（P〈0．05）；ASICs激活组在注射后30min内，癫痫样放电次数增高（1．05±0．07）（P〈0．05）。结论阿米洛利能抑制锂一匹罗卡品诱导的癫痫发作，酸性液体可促进癫痫发作，其作用机制可能与抑制或激活ASICs通道有关。Objective To study the effects of acid-sensor ion channels activation or inhibition on the epileptic discharge in EEG of epilepsy rat model induced by Lithium-pilocarpine. Methods Seizures were intraperitoneally induced in rats with lithium-pilocarpine. The rats were divided into four groups： the placebo group, the ASICs inhibitor group, the positive control group and the ASICs activator group by ad- ministrating intraperitoneally PBS, amiloride, Levetiracetam or acidic liquids, respectively. The number of discharge, and amplitude and interval of discharge were selected as the indicators for comparison. Results Sixty-ninety min after the injection, the number of discharge （0. 69 ± 0.08 ） and the amplitude （0. 70± 0. 16） were reduced in the ASICs inhibitor group, while the interval of discharge was increased （1.46 ± 0. 18 ） （ P 〈 0. 05 ） as compared with the placebo group. In the ASICs activator group, the number of discharge （ 1.05 ±0. 07） was increased 30 min after injection. Conclusion Amiloride can inhibit epilepsy induced by lithium-pilocarpine, while acid liquids exert the opposite effects, which may be concerned with the inhibition or activation of ASICs channel.

关 键 词：匹罗卡品 阿米洛利 酸感受离子通道 癫痫 

分 类 号：R742.1[医药卫生—神经病学与精神病学]