人端粒酶逆转录酶基因在骨巨细胞瘤组织的表达及其临床意义  

Clinical significance of human telomerase reverse transcriptase gene expression in giant cell tumor of bone

作　　者：朱宇[1] 马希峰[1] 来秋山[1] 张明生[1] 巴玉峰[2] 黄涛[1] 陈清汉[1] 

机构地区：[1]郑州大学第二附属医院骨科,450014 [2]郑州大学肿瘤医院(河南省肿瘤医院)

出　　处：《中华实验外科杂志》2012年第8期1584-1585,共2页Chinese Journal of Experimental Surgery

摘　　要：目的观察骨巨细胞瘤组织中人端粒酶逆转录酶（hTERT）基因的表达，并探讨其临床意义。方法选择我院2008年1月至2011年12月病理科保存的标本，标本中骨巨细胞瘤84例、骨质增生骨组织32例、异位骨化组织28例，对照组为正常骨组织20例；采用原位分子杂交技术检测和定位标本中的端粒酶hTERT基因，并用图像分析系统分析其表达水平。结果端粒酶hTERT基因在骨巨细胞瘤组织中的表达阳性率为46．4％（39／84），表达强度与骨巨细胞瘤的分化程度明显相关（P〈0．05），表达水平与肿瘤细胞的分布定位一致；端粒酶hTERT基因在骨质增生、异位骨化和正常骨组织中的表达均为阴性。结论骨巨细胞瘤组织的恶化可能与端粒酶hTERT基因相关。Objective To study the clinical significance of human telomerase reverse transcriptase （hTERT） gene expression in giant cell tumor of bone. Methods By using in situ hybridization （ISH） techniques, the clinical significance of hTERT mRNA expression was tested in 84 cases of giant cell tumor , of bone, 32 cases of hyperostosis, 28 cases of heterotopic ossification and 20 cases of normal bone tissues. These cases were collected from Jan. 2008 to Dec. 2011 in our department. The expression level and the location of hTERT gene were observed by using the image analyzing system. Results hTERT was ex- pressed in 46. 4% （ 39/84 ） of giant cell tumors of bone and it had an obvious correlation with the differen- tiation level of tumor cells （ P 〈 0.05 ）. There was a close correlation between the expression of hTERT and the location of tumor cells, hTERT gene was not detected in 32 cases of hyperostosis, 28 cases of heterotop- ic ossification or 20 eases of normal bone tissues. Conclusion hTERT gene may play an important role in the pathogenesis of giant cell tumors of bone.

关 键 词：骨巨细胞瘤 端粒酶 基因表达 

分 类 号：R738.1[医药卫生—肿瘤]