hUHRF1基因对人肿瘤SKOV-3细胞增殖和凋亡的影响  

Effect of hUHRF1 silencing on proliferation and apoptosis of human cancer cell line SKOV-3

作　　者：邵丽佳[1] 周碧芳[2] 严枫[1] 

机构地区：[1]南京医科大学附属肿瘤医院检验科,210009 [2]南京医科大学附属肿瘤医院妇科,210009

出　　处：《中华实验外科杂志》2012年第8期1589-1591,共3页Chinese Journal of Experimental Surgery

基　　金：国家重点基础研究973计划资助项目（2010CB732400）;国家自然科学基金资助项目（21075055）;江苏省医学领军人才与创新团队项目（LJ201131）

摘　　要：目的观察hUHRF1基因对人肿瘤SKOV-3细胞增殖和凋亡的影响。方法小于扰RNA转染肿瘤SKOV．3细胞；实时荧光定量一聚合酶链反应（RT—qPCR）和Westernblot法分别检测转染前后hUHRF1 mRNA及蛋白的表达水平；细胞计数试剂盒（CCK-8）试验检测细胞增殖；流式细胞术分析细胞凋亡。结果hUHRFlmRNA在转染组表达显著降低（P〈0．01）；转染组、阴性组和空白组蛋白相对表达量分别为0．72±0．42、1．66±0．27和1．62±0．29。干扰后，细胞增殖减少（P〈0．05）；各组别细胞凋亡率分别为（42．320±4．174）％、（17．740±1．786）％和（15．440±1．233）％。结论小干扰RNA沉默技术可以显著抑制肿瘤SKOV-3细胞hUHRFl的表达，有效抑制细胞增殖，并显著诱导细胞凋亡。Objective To discuss the effects of hUHRF1 gene on proliferation and apoptosis effects of human ovarian cancer cell line SKOV-3. Methods Small interfering RNA （siRNA） for hUHRF1 gene sequence was transfected into SKOV-3 cells, Real-time quantitative polymerase chain reaction （RT- qPCR） and Western blotting assays were used to detect the mRNA and protein expression levels of hU- HRF1 respectively before and after siRNA transfection. Cell counting Kit-8 （ CCK-8 ） assay was used to in- vestigate proliferation. Flow cytometry was used to measure apoptosis. Results mRNA expression of hU- HRF1 was significantly decreased in SKOV-3 cells after the transfection of siRNA （ P 〈 O. O1 ）. Relative expression of hUHRF1 protein in transfection group, negative control group and blank group was O. 72 ± 0.42, 1.66 ± O. 27 and 1.62 ± 0. 29 respectively. The proliferation of SKOV-3 cells was inhibited after transfection. Apoptosis rate of SKOV-3 cells in transfection group, negative control group and blank group was （42. 320 ± 4. 174 ） %, （ 17. 740 ± 1. 786 ） % and （ 15.440 ± 1. 233 ） %, respectively. Conclusion Using the siRNA gene silencing techniques, the expression of hUHRF1 is significantly inhibited after gene silencin.g in SKOV-3 cells, which can effectively inhibited SKOV-3 cell proliferation and induced apoptosis.

关 键 词：卵巢癌 RNA干扰 hUHRF1 

分 类 号：R730.5[医药卫生—肿瘤]