血液透析和肾移植患者外周血淋巴细胞PD-L1的表达  

Expression of PD-L1 in peripheral blood lymphocytes from maintenance hemodialysis and kidney transplant patients

作　　者：魏亚非[1,2] 刘永光[1] 郭颖[1] 张建强[1] 顾冬生[1] 赵明[1] 

机构地区：[1]南方医科大学珠江医院,广州市510282 [2]南方医科大学

出　　处：《实用医学杂志》2012年第16期2721-2724,共4页The Journal of Practical Medicine

摘　　要：目的:探讨PD-L1在血液透析和肾移植患者外周血淋巴细胞上的表达及意义。方法:选取2011年1月至2011年9月在我院住院的24例维持性血液透析患者、18例正常肾移植受者作为两个病例组,另外选择10例健康志愿者作为正常对照组。采集三组人群的外周血,运用流式细胞染色技术检测淋巴细胞PD-L1的表达情况。结果:维持性血液透析组淋巴细胞群中CD3+PD-L1+比例明显高于正常肾移植受者组(P<0.05)和正常对照组(P<0.001),且正常肾移植受者组亦高于正常对照组(P<0.05)。而淋巴细胞群中CD3-PD-L1+比例在维持性血液透析组和正常肾移植受者组中表达均高于正常对照组(P<0.001和P<0.05),但维持性血液透析组与正常肾移植受者组之间无统计学差异(P>0.05)。结论:PD-L1在血液透析和肾移植患者外周血淋巴细胞中表达上调,其可能参与了血液透析患者免疫功能低下状态的形成及正常肾移植受者免疫耐受的维持。Objective To investigate PD-L1 expression in peripheral blood lymphocytes from maintenance hemodialysis （MHD） and kidney transplant patients. Methods From January 2011 to September 2011, 24 MHD patients and 18 kidney transplant patients in our hospital and 10 healthy volunteers were enrolled in this study. Flow cytometry was used to detect PD-L1 expression in peripheral blood lymphocytes in these three groups. Results The percentage of CD3＋PD-LI＋cells in lymphocytes in MHD patients [ （8.66±3.28）% ] was much higher than that in the kidney transplant patients [ （6.17±2.08）%, P = 0.014] and was much higher than that in healthy volunteers [ （4.05±1.55 ）%, P = 0.000 ]. This percentage of CD3＋PD-LI cells in lymphocytes in kidney transplant patients was also higher than that in healthy volunteers （P = 0.016）. PD-L1 expression in CD3-1ymphocytes in MHD patients [ （5.31±3.81 ）%] and kidney transplant patients [ （3.22±2.31 ）%] was both higher than that in the healthy control group [ （1.24±0.85）% ,P = 0.000 and P = 0.010], but no significant difference was found between MHD patients and kidney transplant patients （P = 0.095）. Conclusion The up-regulated PD-Llexpression in peripheral blood lymphocytes may be involved in the pathogenesis of low immune function in MHD patients and in the maintenance of immune tolerance in kidney transplant patients.

关 键 词：肾移植 肾透析 淋巴细胞 PD-L1 

分 类 号：R459.5[医药卫生—治疗学]