没食子酸对人正常肝细胞LO2的体外细胞毒性研究  

Research of Gallic Acid on in-vitro Cell Toxicity of Normal Human Hepatocyte LO2

作　　者：严晓莺[1] 陈巨鹏[2] 许冬青[1] 李桂霞[1] 赵凤鸣[1] 徐德国[1] 周韬[1] 王明艳[1] 

机构地区：[1]南京中医药大学基础医学院,江苏南京210046 [2]江苏省中医院,江苏南京210029

出　　处：《辽宁中医杂志》2012年第8期1582-1583,I0001,共3页Liaoning Journal of Traditional Chinese Medicine

摘　　要：目的:探讨抗肿瘤单体没食子酸(GA)对正常人肝细胞(LO2)的体外细胞毒性。方法:采用MTT实验、流式细胞术、Hoechst-33258荧光染色法和电镜检测。结果:GA作用24h后,0,6.25,12.5,25,50,100,200μmol/L用药组出现不同程度的细胞增殖抑制现象,抑制率分别为0,18.2%,25.5%,42.0%,42.5%,51.8%,60.6%;流式细胞仪检测0,25,50,100μmol/L用药组凋亡率分别为1.37%,4.63%,4.64%,12.53%;荧光染色和电镜检测提示100μmol/L组细胞有形态改变,细胞核固缩、碎裂。结论:抗肿瘤单体GA对LO2细胞增殖也有抑制作用,并能诱导LO2细胞凋亡。Objective：To explore in-vitro cell toxicity of anti-tumor drug gallic acid（GA）on normal human hepatocyte（LO2）.Methods：MTT experiment,flow cytometry,Hoechst-33258 fluorescent staining and electron microscope detection were applied.Results：0,6.25,12.5,25,50,100 and 200μmol/L medication groups presented different degrees of proliferation inhibition after GA acted on 24h,and inhibitory rates were 0,18.2%,25.5%,42.0%,42.5%,51.8% and 60.6% respectively;0,25,50 and 100μmol/L medication groups were detected by flow cytometer,whose apoptosis rates were 1.37%,4.63%,4.64% and 12.53% respectively;fluorescent staining and electron microscope detection indicated that cells manifested morphological changes,nuclear pycnosis and fragmentation in 100μmol/L group.Conclusion：Anti-tumor monomer GA also has the inhibitory effect on the proliferation of cell LO2 and can evoke LO2 apoptosis.

关 键 词：没食子酸 肝细胞 毒性 

分 类 号：R285.5[医药卫生—中药学]