黄芪-丹参药对及其组分对肾纤维化梗阻大鼠肾组织Ⅰ、Ⅲ型胶原的影响  

Effect of Astragalus and Salvia's Effective Components and Their Compatibility in Col Ⅰ and Col Ⅲ Expressions of UUO Rat

作　　者：李均[1] 曹轶璇[1] 王冬[1] 阳小敏[1] 周萍[1] 

机构地区：[1]遵义医学院第五附属(珠海)医院,广东珠海519100

出　　处：《辽宁中医杂志》2012年第8期1640-1642,共3页Liaoning Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金项目(30960490);贵州省社会攻关项目(黔科合SY字[2009]3078);贵州省省长基金项目[黔省专合字(2010)69]

摘　　要：目的:观察单侧输尿管梗阻(UUO)大鼠肾组织Ⅰ、Ⅲ型胶原含量,探讨黄芪丹参药对及其有效成分对肾纤维化的影响。方法:66只SD大鼠随机分为7组,正常组、模型组、福辛普利组(用蒙诺化学名取代商品名)、丹参总酚酸组、黄芪总皂苷组、黄芪丹参颗粒剂配伍1∶1组(简称颗粒配伍组)、黄芪丹参组分配伍1∶1组(简称组分配伍组)。各组手术后第15天取出梗阻侧肾脏,组织经4%多聚甲醛固定24h,然后常规脱水包埋制成石蜡切片。部分进行HE、Masson病理染色,光学显微镜下观察;另一部分进行免疫组化实验,观察Ⅰ、Ⅲ型胶原沉积情况。结果:各组肾脏病理损害、胶原沉积及免疫组化结果表现一致,均以模型组最明显;表达最轻的为福辛普利组及组分配伍组。结论:UUO大鼠肾组织胶原沉积明显,黄芪丹参药对及其有效成分干预后在一定程度上能改善肾纤维化。Objective：To observe the changes of UUO rat＇s Col Ⅰ and Col Ⅲ expressions,the influence of Astragalus and Salvia＇s effective components and their compatibility on renal fibrosis were investigated.Methods：66 SD rats were randomly divided into 7 groups,normal group,model group,fosinopril group,salvianolic acids group,astragalus saponins group,granules compatibility of Astragalus and Salvia（1∶ 1）group,components combination of Astragalus and Salvia（1∶ 1）group.15 days after the surgery,the obstructed kidney in each group was taken out and fixed by 4% paraform.The nephridial tissues were made into paraffin section by dehydration and embedding which were used for HE,Masson pigmentation and immunohistochemistry experiment.Results：The pathological lesion and collagen deposition in each group were accord with the result of immunohistochemistry experiment.The model group expressed the most serious pathological changes while the fosinopril group and granules compatibility of Astragalus and Salvia（1∶ 1） group showed pathological changes to the most minor.Conclusion：UUO deposition of collagen in renal tissue was obvious.Astragalus and its active ingredient of Salvia drug intervention can alleviate renal fibrosis at a certain degree.

关 键 词：输尿管梗阻 黄芪 丹参 有效成分 Ⅰ型胶原 Ⅲ型胶原 

分 类 号：R-332[医药卫生]