一株产生氨氮和亚硝酸盐氮菌的筛选鉴定及特性研究  

Isolation,Identification and the Characteristics of a Strain with Ability of Producing Ammonia and Nitrite

作　　者：傅罗琴[1] 邓斌[1] 郑佳佳[1] 张小平[1] 梁权[1] 李卫芬[1] 

机构地区：[1]浙江大学动物科学学院,杭州310058

出　　处：《农业环境科学学报》2012年第8期1616-1623,共8页Journal of Agro-Environment Science

基　　金：国家重点基础研究发展规划(973)项目(2009CB118705);国家水体污染控制与治理重大专项项目(2008ZX07101-006)

摘　　要：从4个草鱼池塘中分离和定性筛选获得29株能够产生氨氮和亚硝酸盐氮的菌株。通过对编号为C95的菌株进行菌落形态学观察和16S rDNA序列分析,表明该菌株为革兰氏阴性杆状菌,与寡养单胞菌属(Stenotrophomonas sp.)的同源性达98%。采用单因素多水平试验对菌株的产氨氮和产亚硝酸盐氮特性进行研究发现:(1)氮源、碳源、温度和摇床转速都能显著影响菌株的生长及产生氨氮和亚硝酸盐氮的含量,但pH(5～9)对其无显著影响(P>0.05);(2)该菌株生长及产生氨氮和亚硝酸盐氮最适宜的培养基以及培养条件为:LB、pH 5～9、25℃、150 r.min-1。由C95作为指示菌株筛选得到SC01、SC07两株(2/33)去除氨氮和亚硝酸盐氮效果较好的菌株。因此,C95可作为筛选具有降氨氮和亚硝酸盐氮功能的有益菌的指示菌株。Twenty nine strains with ability of producing ammonia and nitrite were isolated from culture water of 4 grass carp ponds through qualitative filtration. One of them, named C95, was identified as a Gram negative, rod-shaped strain exhibiting the highest sequence homolo- gy to Stenotrophomonas sp. （98%） according to its colony morphological properties and the 16S rRNA sequence analysis. Characterization of growth and ammonia nitrogen and nitrite nitrogen production revealed that： （1）nitrogen resource, carbon resource, temperature and rotation speed could significantly affect the strain properties; （2）the optimal substrate, pH, temperature and rotation speed were LB, pH5 -9, 25 ℃, 150 r· min-1, respectively. Two strains（ 2/33 ） named SC01 and SC07 that can degrade ammonia and nitrite better were isolated by the strain C95 as an indicator strain for the beneficial bacteria with ammonia and nitrite nitrogen removal function, which has potential application val- ue to improve the quality of culture water.

关 键 词：异养硝化菌 氨氮 亚硝酸盐氮 寡养单胞菌属 

分 类 号：X172[环境科学与工程—环境科学]