脉络瘀滞型血管性痴呆动物模型研制的实验研究  

Vascular Dementia Animal Model With Types of Collaterals Siltation and Stagnant: Model Establishment and Experimental Study

作　　者：万睿[1] 辜尊涛[1] 罗小泉[1] 

机构地区：[1]江西中医学院,南昌330004

出　　处：《江西中医学院学报》2012年第1期58-60,共3页Journal of Jiangxi College of Traditional Chinese Medicine

基　　金：江西省自然科学基金(20114BAB205072)

摘　　要：目的:初步建立了脉络瘀滞型血管性痴呆动物模型,为现代中医药探明血管性痴呆发病机制及合理用药提供符合中医理论的病证结合动物模型。方法:采用脂肪乳灌胃合并脑缺血再灌注制作脉络瘀滞型血管性痴呆动物模型,以Morris水迷宫检测大鼠的学习记忆能力,同时测定各组大鼠脑内AchE活性及MDA的含量,观察比较该模型与单纯的脑缺血再灌注血管性痴呆动物模型同正常动物之间存在的差异。结果:灌服脂肪乳合并脑缺血再灌注所造的模型较单纯脑缺血再灌注血管性痴呆动物模型可造成更加明显学习记忆障碍,且脑组织中AchE及MDA的含量明显增高。结论:该模型更符合中医理论,可作为今后中医药研究血管性痴呆的理想模型。Objective：To establish vascular dementia animal model with types of collaterals siltation and stagnant,provide the animal model of combination of diseases with differentiation of syndromes which conforms to traditional chinese medicine and find out vascular dementia’s pathogenesis and rational administration.Methods：To establish the vascular dementia animal model with types of collaterals siltation and stagnant,the rats were intragastric infused of fat galacta combine with ischemia-reperfusion.Then test the ability of learning and memory of rats by Morris water maze.And determine the intracephalic AcheE’s activity and the content of MDA of each rat groups,observe the different between the model and simple ischemia-reperfusion vascular dementia animal model and nomal animal.Results：compared with simple ischemia-reperfusion vascular dementia animal model,the model may obviously cause learning memory disorders,and increase the content of rats ’s intracephalic AcheE and MDA.Conclusion：The model was closer to traditional chinese medicine,and can be seen as a more stable and reliable animal model of vascular dementia.

关 键 词：脉络瘀滞 行为学障碍 血管性痴呆 动物模型 

分 类 号：R-332[医药卫生]