A Facile Approach to Fabricate Water-soluble Au-Fe3O4 Nanoparticle for Liver Cancer Cells Imaging  

作　　者：梁重时 吴献荣 谢叶归 刘顺英 

机构地区：[1]Shanghai Engineering Research Center for Molecular Theraputics and New Drug Development,East China Normal University,Shanghai 200062,China

出　　处：《Chinese Journal of Chemistry》2012年第7期1387-1392,共6页中国化学（英文版）

摘　　要：Au-Fe3O4 nanoparticles were widely used as nanoplatforms for biologic applications through readily further functionalization. Dopamine （DA）-coated superparamagnetic iron oxide （SPIO） nanoparticles （DA@Fe3O4） have been successfully synthesized using a one-step process by modified coprecipitation method. Then 2--3 nm gold nanoparticles were easily conjugated to DA@Fe3O4 nanoparticles by the electrostatic force between gold nanoparti- cles and amino groups of dopamine to afford water-soluble Au-Fe3O4 hybrid nanoparticles. A detailed investigation by dynamic light scatting （DLS）, transmission electron microscopy （TEM）, fourier transform infrared （FT-IR） and X-ray diffraction （XRD） were performed in order to characterize the physicochemical properties of the hybrid nanoparticles. The hybrid nanoparticles were easily functionalized with a targeted small peptide A54 （AGKGTPSLETTP） and fluorescence probe fluorescein isothiocyanate （FITC） for liver cancer cell BEL-7402 imaging. This simple approach to prepare hybrid nanoparticles provides a facile nanoplatform for muti-functional derivations and may be extended to the immobilization of other metals or bimolecular on SPIO surface.Au-Fe3O4 nanoparticles were widely used as nanoplatforms for biologic applications through readily further functionalization. Dopamine （DA）-coated superparamagnetic iron oxide （SPIO） nanoparticles （DA@Fe3O4） have been successfully synthesized using a one-step process by modified coprecipitation method. Then 2--3 nm gold nanoparticles were easily conjugated to DA@Fe3O4 nanoparticles by the electrostatic force between gold nanoparti- cles and amino groups of dopamine to afford water-soluble Au-Fe3O4 hybrid nanoparticles. A detailed investigation by dynamic light scatting （DLS）, transmission electron microscopy （TEM）, fourier transform infrared （FT-IR） and X-ray diffraction （XRD） were performed in order to characterize the physicochemical properties of the hybrid nanoparticles. The hybrid nanoparticles were easily functionalized with a targeted small peptide A54 （AGKGTPSLETTP） and fluorescence probe fluorescein isothiocyanate （FITC） for liver cancer cell BEL-7402 imaging. This simple approach to prepare hybrid nanoparticles provides a facile nanoplatform for muti-functional derivations and may be extended to the immobilization of other metals or bimolecular on SPIO surface.

关 键 词：DOPAMINE GOLD superparamagnetic iron oxide hybrid nanoparticles cell imaging 

分 类 号：Q95-33[生物学—动物学] TQ110[化学工程—无机化工]