HPLC-UV法同时测定麻黄中5种麻黄生物碱的含量  

Simultaneous Determination of Five Ephedrine Alkaloids in Ephedrae Herba by HPLC-UV

作　　者：陈燕[1] 兰树敏[1] 林壮民[1] 梅清华[1] 高玲[1] 罗佳波[2] 

机构地区：[1]广东省第二人民医院药学部,广东广州510317 [2]南方医科大学中医药学院,广东省中药制剂重点实验室,广东广州510515

出　　处：《今日药学》2012年第7期388-391,397,共5页Pharmacy Today

基　　金：国家自然科学基金资助项目(编号:30772756)

摘　　要：目的建立同时分离检测麻黄中麻黄碱(Ephedrine)、伪麻黄碱(Pseudoephedrine)、去甲麻黄碱(Norephedrine)、去甲伪麻黄碱(Norpseudoephedrine)和甲基麻黄碱(Methylephedrine)的含量测定方法。方法采用HPLC-UV法,色谱柱:苯基柱(Alltima Phenyl,250mm×4.6 mm,5μm);流动相:0.02 mol/L磷酸二氢钾溶液-乙腈(96∶4);流速:0.6 ml/min;检测波长:210 nm;柱温:25℃。结果 5种生物碱在13.5 min内即可达到完全分离,E在2.000 8～40.016 0μg/ml线性关系良好;PE在1.003 6～20.072 0μg/ml线性关系良好;NME在0.199 2～3.984 0μg/ml线性关系良好;NMP在0.200 0～4.00 0μg/ml线性关系良好;ME在0.200 4～4.008 0μg/ml线性关系良好。各生物碱的平均回收率均在92%～104%(RSD≤5.76%)。结论本方法可操作性强,简便快速,分离效果好,重现性好,可为麻黄及含麻黄的中西药制剂提供有效的检测手段。Objective To establish HPLC-UV method to simultaneously determine the contents of ephedrine, pseudoephedrine, norephedrine, norpseudoephedrine, and methylephedrine in Ephedrae Herba. Methods Alhima Phenyl column （250 mm ×4.6 mm, 5 um） maintained at 25 ℃ with 0.02 mol/L KH2PO4-acetonitrile （96： 4） as the mobile phase was used. The flow rate was 0.6 ml/min and the detective wavelength was at 210 nm. Results The 5 ephedrine alkaloids were separated in 13.5 min. The calibration curve for ephedrine, pseudoephedrine, norephedrine, norpseudoephedrine, and methylephedrine was linear in the range of 2. 000 8 - 40.016 0 ug/ml, 1. 003 6 - 20. 072 0 ug/ml,0. 199 2 - 3. 984 0 ug/ml,0.200 0 -4. 000 0 ug/ml and 0. 200 4 -4. 008 0 ug/ml, respectively. The average recovery was among 92% - 104% （RSD ≤5.76% ）. Conclusion This method shows excellent operability, separation, linearity, specificity, accuracy, precision. It is suitable for the separation and quantization of ephedrines in finished product containing Ephedra sinica or ephedrine alkaloids.

关 键 词：麻黄 麻黄碱 伪麻黄碱 去甲麻黄碱 去甲伪麻黄碱 甲基麻黄碱 HPLC-UV 

分 类 号：R286.02[医药卫生—中药学]