直肠癌中磷酸酶和张力蛋白同源基因及磷脂酰肌醇3激酶的表达与临床病理特征的相关性  

Correlation of phosphatase and tensin homolog gene and 1-phosphatidylinositol 3-kinase expression with clinical pathology in rectal cancer

作　　者：刘斌[1] 宫爱民[1] 周永娜[1] 赵长林[2] 杨娟[2] 袁俊波[2] 

机构地区：[1]大连大学附属新华医院肛肠科二病房,116021 [2]大连大学附属新华医院肿瘤科一病房,116021

出　　处：《中国医师进修杂志》2012年第23期1-4,共4页Chinese Journal of Postgraduates of Medicine

摘　　要：目的探讨磷酸酶和张力蛋白同源基因（PTEN）及磷脂酰肌醇3激酶（P13K）在直肠癌中的表达与临床病理特征的相关性。方法应用免疫组织化学染色MaxVisionTM法检测60例直肠癌、30例直肠腺瘤和10例正常直肠黏膜组织标本中PTEN及P13K的表达情况，并对其表达水平与直肠癌临床病理特征之间的关系进行相关性分析。结果直肠癌组织PTEN阳性表达率明显低于正常直肠黏膜组织和直肠腺瘤组织[43．3％（26／60）比100．0％（10／10）和93．3％（28／30），P〈0．05]，直肠癌组织P13K阳性表达率明显高于正常直肠黏膜组织和直肠腺瘤组织[75．0％（45／60）比30．O％（3／10）和33．3％（10／30），P〈0．05]。PTEN及P13K在直肠癌组织中的表达水平与患者术前癌胚抗原、分化程度、TNM分期、淋巴结转移和远处转移有关（P〈0．01或〈0．05），而与患者年龄、性别、肿瘤位置、肿瘤形态无明显相关性（P〉0．05）。相关分析结果表明，P13K与PTEN阳性表达呈显著负相关（r=-0．269，P=0．023）。结论直肠癌中P13K高表达及PTEN低表达，与直肠癌的侵袭和转移密切相关，监测两者的表达对判定直肠癌生物学行为有一定的价值。Objective To investigate the expression of phosphatase and tensin homolog gene （PTEN） and 1-phosphatidylinositol 3-kinase （PI3K） in rectal cancer and its correlation with clinical pathology. Methods MaxVisionTM immunohistochemical method was used to detect the expressions of PTEN and PI3K in 60 rectal cancer tissues,30 adenoma tissues and 10 normal rectal tissues, and the correlation between the expression levels and its clinicopathologic characteristics was discussed. Results The positive expression rate of PTEN in rectal cancer tissue was significantly lower than that in normal rectal tissue and adenoma tissue [43.3% （26/60） vs. 100.0% （ 10/10）,93.3% （28/30）,P 〈 0.05]. The positive expression rate of PI3K in rectal cancer tissue was significantly higher than that in normal rectal tissue and adenoma tissue [75.0% （45/60） vs. 30.0% （3/10） ,33.3% （10/30） ,P 〈 0.05]. The positive expression of PTEN and PI3K were correlated with carcinoembryonic antigen, degree of cell differentiation, TNM stage, lymph node metastasis and distant metastasis（P 〈 0.01 or 〈 0.05 ）, but had no correlation with age, gender, neoplasm location, neoplasm appearance （P 〉 0.05）. The correlated analysis showed that the positive expression of PTEN had negative correlation with PI3K （r = -0.269,P = 0.023 ）. Conclusions The high expression of PI3K and down regulation of PTEN in rectal cancer are closely related to its invasion anti metastasis. There are some values to monitor PTEN and PI3K expression for determining biological behavior of rectal cancer.

关 键 词：直肠肿瘤 1-磷脂酰肌醇3-激酶 磷酸酶和张力蛋白同源基因 免疫组织化学 

分 类 号：R735.37[医药卫生—肿瘤]